Tvarijonaviciute Asta, Ceron Jose J, Tecles Fernando
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain.
Vet Clin Pathol. 2013 Jun;42(2):207-11. doi: 10.1111/vcp.12032. Epub 2013 Apr 1.
Obesity is the most common nutritional disorder in today's dog population and the major risk factor for a number of related diseases. However, the exact pathogenesis of obesity-related complications is not always clear. In people, butyrylcholinesterase (BChE) is suspected to be involved in lipoprotein metabolism and has also been associated with the pathogenesis of inflammatory disease, one of the potential complications related to obesity.
The aim of the study was to evaluate the effect of experimentally induced weight loss on BChE and acetylcholinesterase (AChE) in obese dogs to elucidate the possible relationship between these 2 enzymes and obesity.
Six obese intact female Beagle dogs were allocated to a weight loss program for 3 months. BChE was measured in serum samples using butyrylcholine as substrate, whereas AChE was measured in whole blood after inhibition of BChE with ethopropazine and using acetylcholine as a substrate.
After rapid weight loss serum BChE activities were statistically significantly lower (P < .05), whereas AChE activities were higher (P < .01). There was a positive correlation between serum BChE activity and concentrations of total cholesterol (TCHOL, P < .001), low-density lipoprotein-cholesterol (LDL-C, P < .001), and triglycerides (P < .05). A negative correlation was detected between serum BChE and AChE activities (P < .0001), and between AChE activity and serum levels of TCHOL (P < .01), LDL-C (P < .01) and high-density lipoprotein-cholesterol (P < .05).
Short-term weight loss in obese intact female Beagle dogs resulted in opposite effects in 2 cholinesterase isoenzyme activities, namely lower BChE and higher AChE activities.
肥胖是当今犬类最常见的营养失调问题，也是多种相关疾病的主要风险因素。然而，肥胖相关并发症的确切发病机制并不总是明确的。在人类中，丁酰胆碱酯酶（BChE）被怀疑参与脂蛋白代谢，并且也与炎症性疾病的发病机制有关，炎症性疾病是与肥胖相关的潜在并发症之一。
本研究的目的是评估实验性诱导体重减轻对肥胖犬体内BChE和乙酰胆碱酯酶（AChE）的影响，以阐明这两种酶与肥胖之间可能的关系。
将6只肥胖的未绝育雌性比格犬分配到一个为期3个月的体重减轻计划中。使用丁酰胆碱作为底物测定血清样本中的BChE，而在用乙丙嗪抑制BChE后，使用乙酰胆碱作为底物测定全血中的AChE。
快速体重减轻后，血清BChE活性在统计学上显著降低（P <.05），而AChE活性升高（P <.01）。血清BChE活性与总胆固醇（TCHOL，P <.001）、低密度脂蛋白胆固醇（LDL-C，P <.001）和甘油三酯（P <.05）浓度之间存在正相关。血清BChE和AChE活性之间（P <.0001），以及AChE活性与血清TCHOL水平（P <.01）、LDL-C（P <.01）和高密度脂蛋白胆固醇（P <.05）之间检测到负相关。
肥胖的未绝育雌性比格犬短期体重减轻导致两种胆碱酯酶同工酶活性产生相反的影响，即BChE活性降低而AChE活性升高。
