State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, China.
FEMS Microbiol Ecol. 2013 Aug;85(2):358-68. doi: 10.1111/1574-6941.12125. Epub 2013 Apr 18.
Sorangium cellulosum, a cellulolytic myxobacterium, is capable of producing a variety of bioactive secondary metabolites. Epothilones are anti-eukaryotic secondary metabolites produced by some S. cellulosum strains. In this study, we analyzed interactions between 12 strains of S. cellulosum consisting of epothilone-producers and non-epothilone producers isolated from two distinct soil habitats. Co-cultivation on filter papers showed that different Sorangium strains inhibited one another's growth, whereas epothilone production by the producing strains changed markedly for most (73%) pairwise mixtures. Using a quantitative polymerase chain reaction, we demonstrated that the expression of epothilone biosynthetic genes in the epothilone producers typically changed significantly when these bacteria were mixed with non-producing strains. The results indicated that intraspecies interactions between different S. cellulosum strains not only inhibited the growth of partners, but also could change epothilone production.
索拉金菌是一种能产生多种生物活性次级代谢产物的纤维素分解菌，表二酮类化合物是某些索拉金菌菌株产生的抗真核生物次级代谢产物。在这项研究中，我们分析了由来自两个不同土壤生境的产表二酮类化合物菌株和非产表二酮类化合物菌株组成的 12 株索拉金菌之间的相互作用。在滤纸上的共培养表明，不同的索拉金菌菌株相互抑制生长，而对于大多数（73%）的成对混合物，产表二酮类化合物菌株的表二酮类化合物的产生明显改变。使用定量聚合酶链反应，我们证明当这些细菌与非产菌株混合时，产表二酮类化合物的细菌中表二酮类化合物生物合成基因的表达通常会显著变化。结果表明，不同索拉金菌菌株之间的种内相互作用不仅抑制了伙伴的生长，而且还可以改变表二酮类化合物的产生。
