Regentin R, Frykman S, Lau J, Tsuruta H, Licari P
Department of Process Sciences, Kosan Biosciences, 3832 Bay Center Place, Hayward, CA 94545, USA.
Appl Microbiol Biotechnol. 2003 Jun;61(5-6):451-5. doi: 10.1007/s00253-003-1263-1. Epub 2003 Mar 6.
Fermentation media with different initial concentrations of ammonium and phosphate salts were used to study the inhibitory effects of those ions on growth and production of epothilone in Sorangium cellulosum and Myxococcus xanthus. The native epothilone producer, S. cellulosum was more sensitive to ammonium and phosphate than the heterologous producer, M. xanthus. An ammonium concentration of 12 mM reduced epothilone titers by 90% in S. cellulosum but by only 40% in M. xanthus. When 5 mM phosphate was added to the medium, production in both strains was 60% lower. Higher phosphate concentrations had little additional effect on M. xanthus titers, but epothilone production with 17 mM extra-cellular phosphate in S. cellulosum was 95% lower than in the control condition. The effect of iron supplementation to the fermentation medium was also investigated. Both strains showed best production with 20 microM iron added to the medium.
使用含有不同初始浓度铵盐和磷酸盐的发酵培养基，研究这些离子对纤维堆囊菌和黄色粘球菌中埃坡霉素生长和产生的抑制作用。天然埃坡霉素产生菌纤维堆囊菌比异源产生菌黄色粘球菌对铵和磷酸盐更敏感。12 mM的铵浓度使纤维堆囊菌中的埃坡霉素效价降低90%，但在黄色粘球菌中仅降低40%。当向培养基中添加5 mM磷酸盐时，两种菌株的产量均降低60%。更高的磷酸盐浓度对黄色粘球菌效价几乎没有额外影响，但在纤维堆囊菌中，胞外磷酸盐浓度为17 mM时的埃坡霉素产量比对照条件下低95%。还研究了向发酵培养基中补充铁的效果。两种菌株在向培养基中添加20 microM铁时产量最佳。
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