通过基因掺入金属螯合非天然氨基酸实现荧光蛋白生色团的显著扩展。 - Suppr

Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid.

作者信息

Liu Xiaohong, Li Jiasong, Hu Cheng, Zhou Qing, Zhang Wei, Hu Meirong, Zhou Juanzuo, Wang Jiangyun

机构信息

Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.

出版信息

Angew Chem Int Ed Engl. 2013 Apr 26;52(18):4805-9. doi: 10.1002/anie.201301307. Epub 2013 Mar 28.

DOI:10.1002/anie.201301307

PMID:23554162

Abstract

摘要

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Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid.通过基因掺入金属螯合非天然氨基酸实现荧光蛋白生色团的显著扩展。

Angew Chem Int Ed Engl. 2013 Apr 26;52(18):4805-9. doi: 10.1002/anie.201301307. Epub 2013 Mar 28.

Role of lysine-256 in Citrobacter freundii tyrosine phenol-lyase in monovalent cation activation.赖氨酸-256在弗氏柠檬酸杆菌酪氨酸酚裂解酶单价阳离子激活中的作用。

Biochemistry. 2004 Nov 16;43(45):14412-9. doi: 10.1021/bi0484062.

Tyrosine phenol-lyase: the role of the coenzyme-binding residue Ser-254 in catalysis.酪氨酸酚裂解酶：辅酶结合残基Ser-254在催化中的作用。

Dokl Biochem Biophys. 2003 Jul-Aug;391:225-8. doi: 10.1023/a:1025117626374.

Crystal Structures of Wild-Type and F448A Mutant Citrobacter freundii Tyrosine Phenol-Lyase Complexed with a Substrate and Inhibitors: Implications for the Reaction Mechanism.野生型和 F448A 突变型弗氏柠檬酸杆菌酪氨酸酚裂解酶与底物和抑制剂复合物的晶体结构：对反应机制的启示。

Biochemistry. 2018 Oct 30;57(43):6166-6179. doi: 10.1021/acs.biochem.8b00724. Epub 2018 Oct 12.

Aspartic acid 214 in Citrobacter freundii tyrosine phenol-lyase ensures sufficient C--H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site.弗氏柠檬酸杆菌酪氨酸酚裂解酶中的天冬氨酸214可确保外部醛亚胺中间体具有足够的C-H酸度，并使辅因子在活性位点具有正确的方向。

Biochim Biophys Acta. 2006 Jul;1764(7):1268-76. doi: 10.1016/j.bbapap.2006.05.001. Epub 2006 May 16.

The role of glutamic acid-69 in the activation of Citrobacter freundii tyrosine phenol-lyase by monovalent cations.谷氨酸-69在一价阳离子激活弗氏柠檬酸杆菌酪氨酸酚裂解酶中的作用。

Biochemistry. 2000 Jul 25;39(29):8546-55. doi: 10.1021/bi000063u.

Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer.

Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10261-5. doi: 10.1002/anie.201204962. Epub 2012 Aug 31.

The crystal structure of Citrobacter freundii tyrosine phenol-lyase complexed with 3-(4'-hydroxyphenyl)propionic acid, together with site-directed mutagenesis and kinetic analysis, demonstrates that arginine 381 is required for substrate specificity.弗氏柠檬酸杆菌酪氨酸酚裂解酶与3-(4'-羟基苯基)丙酸复合的晶体结构，结合定点突变和动力学分析，表明精氨酸381是底物特异性所必需的。

Biochemistry. 1997 May 27;36(21):6502-10. doi: 10.1021/bi962917+.

Inhibition of tyrosine phenol-lyase from Citrobacter freundii by 2-azatyrosine and 3-azatyrosine.2-氮杂酪氨酸和3-氮杂酪氨酸对弗氏柠檬酸杆菌酪氨酸酚裂解酶的抑制作用。

Biochemistry. 2001 Dec 11;40(49):14862-8. doi: 10.1021/bi015707s.

Binding of phenol and analogues to alanine complexes of tyrosine phenol-lyase from Citrobacter freundii: implications for the mechanisms of alpha,beta-elimination and alanine racemization.苯酚及其类似物与弗氏柠檬酸杆菌酪氨酸酚裂解酶的丙氨酸复合物的结合：对α,β-消除和丙氨酸消旋机制的启示

Biochemistry. 1993 Nov 2;32(43):11591-9. doi: 10.1021/bi00094a016.

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Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid.

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