Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, N-68588, USA.
The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, NE-68588, USA.
Chembiochem. 2023 May 2;24(9):e202300039. doi: 10.1002/cbic.202300039. Epub 2023 Mar 30.
Protein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co-translational installation and studies of PTMs through noncanonical amino acid (ncAA) mutagenesis. We begin by reviewing types of PTM that have been installed by GCE with a focus on modifications of tyrosine, serine, threonine, lysine, and arginine residues. We also discuss examples of applying these methods in biological studies. Finally, we end the piece with a short discussion on the challenges and the opportunities of the field.
蛋白质翻译后修饰（PTMs）在调节细胞活动中起着关键作用。在这里，我们提供了一个综述，介绍了遗传密码扩展（GCE）方法在通过非天然氨基酸（ncAA）诱变进行共翻译安装和 PTM 研究中的应用。我们首先回顾了通过 GCE 安装的各种 PTM，重点介绍了酪氨酸、丝氨酸、苏氨酸、赖氨酸和精氨酸残基的修饰。我们还讨论了将这些方法应用于生物学研究的实例。最后，我们简短讨论了该领域的挑战和机遇。
