Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA.
J Infect Dis. 2013 Jun 15;207(12):1807-16. doi: 10.1093/infdis/jit118. Epub 2013 Apr 3.
Hematopoietic progenitor cells (HPCs) in the bone marrow of human immunodeficiency virus (HIV)-infected individuals have been proposed as a persistent reservoir of virus. However, some studies have suggested that HIV genomes detected in HPCs arise from T-cell contamination.
CD133-sorted HPCs and CD133-depleted bone marrow cells were purified from bone marrow specimens obtained from 11 antiretroviral-treated donors in whom the HIV load had been <48 copies/mL for at least 6 months. CD133 and CD3 expression on the cells was assessed by flow cytometry. HIV DNA was quantified by real-time polymerase chain reaction analysis.
HIV genomes were detected in CD133-sorted samples from 6 donors, including 2 in whom viral loads were undetectable for >8 years. CD3(+) T cells represented <1% of cells in all CD133-sorted samples. For 5 of 6 CD133-sorted samples with detectable HIV DNA, the HIV genomes could not be explained by contaminating CD3(+) T cells. Donors with detectable HIV DNA in HPCs received their diagnosis significantly more recently than the remaining donors but had had undetectable viral loads for similar periods.
HIV genomes can be detected in CD133-sorted cells from a subset of donors with long-term viral suppression and, in most cases, cannot be explained by contamination with CD3(+) T cells.
人们提出，骨髓中的造血祖细胞（HPC）是 HIV 感染者体内病毒的持续储存库。然而，一些研究表明，在 HPC 中检测到的 HIV 基因组源自 T 细胞污染。
从 11 名接受抗病毒治疗的供体的骨髓标本中纯化了 CD133 分选的 HPC 和 CD133 耗尽的骨髓细胞，这些供体的 HIV 载量已 <48 拷贝/mL 至少 6 个月。通过流式细胞术评估细胞上的 CD133 和 CD3 的表达。通过实时聚合酶链反应分析定量 HIV DNA。
在 6 名供体的 CD133 分选样本中检测到了 HIV 基因组，其中包括 2 名病毒载量不可检测的患者 >8 年。所有 CD133 分选样本中的 CD3(+) T 细胞均<1%。对于 5 份可检测到 HIV DNA 的 CD133 分选样本，HIV 基因组不能用污染的 CD3(+) T 细胞来解释。在 HPC 中可检测到 HIV DNA 的供体诊断时间明显晚于其余供体，但已达到相似的病毒载量不可检测时间。
在长期病毒抑制的供体亚群中可从 CD133 分选的细胞中检测到 HIV 基因组，并且在大多数情况下，不能用 CD3(+) T 细胞的污染来解释。
