Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China, Fax: +86 10 82612629.
Adv Healthc Mater. 2013 Sep;2(9):1246-51. doi: 10.1002/adhm.201200414. Epub 2013 Apr 4.
Doxorubicin, together with the modified polysaccharide (alginate dialdehyde), was used as a wall material to fabricate microcapsules through self-cross-linking by a template method. The microcapsules as-prepared are pH-responsive. Relevant scanning electronic microscopy, atom force microscopy and confocal laser scanning microscopy confirm the morphology of the uniform microcapsules. The spectroscopic results show that the microcapsules are assembled through electrostatic interaction and Schiff's base covalent bonding. Doxorubicin can be released sustainably from the capsules in buffer solution at a lower pH value. The cellular uptake of the microcapsules and drug release induced by acidic microenvironment are time-dependent processes. The cell cytotoxicity experiments in vitro demonstrate that the doxorubicin-based microcapsules have high efficiency to kill the cancer cells.
多柔比星与改性多糖（海藻酸钠二醛）一起用作壁材，通过模板法自交联制备微胶囊。所制备的微胶囊具有 pH 响应性。相关的扫描电子显微镜、原子力显微镜和共聚焦激光扫描显微镜证实了均匀微胶囊的形态。光谱结果表明，微胶囊通过静电相互作用和席夫碱共价键组装。多柔比星可以在较低 pH 值的缓冲溶液中从胶囊中持续释放。微胶囊的细胞摄取和酸性微环境诱导的药物释放是时间依赖性过程。体外细胞毒性实验表明，基于多柔比星的微胶囊具有高效杀伤癌细胞的能力。
