Tinkov Alexey A, Ajsuvakova Olga P, Shehtman Alexandr M, Boev Viktor M, Nikonorov Alexandr A
Department of Biochemistry, Orenburg State Medical Academy, Orenburg, Russia ; Interdepartmental Biochemical Laboratory, Orenburg State Medical Academy, Orenburg, Russia.
Interdiscip Toxicol. 2012 Sep;5(3):127-32. doi: 10.2478/v10102-012-0021-6.
The aim of the present study was to assess the effect of iron and copper consumption on weight gain and development of oxidative stress in adipose tissue of rats. Control rats obtained pure drinking water. Iron-treated groups of animals obtained FeSO4•12H2O with drinking water in concentrations of 3 and 6 mg/l, while copper-treated rats obtained CuSO4 in concentrations of 4.88 and 9.76 mg/l. The animals of the 6th group received a mixture of FeSO4•12H2O and CuSO4 in the respective concentrations of 3 and 4.88 mg/l in drinking water. All animals received a standard chow. The final weight of rats from all the experimental groups, especially in those obtaining the combination of iron and cooper, exceeded the control values. Maximal weight of fat pads was observed in animals receiving drinking water with 3 mg/l FeSO4•12H2O, 4.88 and 9.76 mg/l CuSO4, and the mixture of FeSO4•12H2O and CuSO4. The maximal intensity of free radical processes, as estimated by the concentration of fluorescent modified amino acids and the intensity of chemiluminescence in adipose tissue homogenates, was observed in rats obtaining iron in the concentration of 3 mg/l in the drinking water.
本研究的目的是评估铁和铜的摄入量对大鼠脂肪组织体重增加及氧化应激发展的影响。对照组大鼠饮用纯净水。铁处理组动物饮用含浓度为3毫克/升和6毫克/升硫酸亚铁·12水合物的水，而铜处理组大鼠饮用含浓度为4.88毫克/升和9.76毫克/升硫酸铜的水。第6组动物饮用含浓度分别为3毫克/升硫酸亚铁·12水合物和4.88毫克/升硫酸铜的混合水。所有动物均喂食标准饲料。所有实验组大鼠的最终体重，尤其是摄入铁和铜组合的那些组，超过了对照组的值。在饮用含3毫克/升硫酸亚铁·12水合物、4.88毫克/升和9.76毫克/升硫酸铜以及硫酸亚铁·12水合物与硫酸铜混合物的水中的动物身上，观察到脂肪垫的最大重量。通过脂肪组织匀浆中荧光修饰氨基酸的浓度和化学发光强度估算，在饮用含3毫克/升铁的水中的大鼠身上观察到自由基过程的最大强度。
