Djurendic-Brenesel Maja, Pilija Vladimir, Mimica-Dukic Neda, Budakov Branislav, Cvjeticanin Stanko
Institute of Forensic Medicine, Clinical Center Vojvodina, Serbia.
Interdiscip Toxicol. 2012 Dec;5(4):173-8. doi: 10.2478/v10102-012-0029-y.
The present study examined regional distribution of opiate alkaloids from seized heroin in brain regions of experimental animals in order to select parts with the highest content of opiates. Their analysis should contribute to resolve causes of death due to heroin intake. The tests were performed at different time periods (5, 15, 45 and 120 min) after male and female Wistar rats were treated with seized heroin. Opiate alkaloids (codeine, morphine, acetylcodeine, 6-acetylmorphine and 3,6-diacetylmorphine) were quantitatively determined in brain regions known for their high concentration of µ-opiate receptors: cortex, brainstem, amygdala and basal ganglia, by using gas chromatography-mass spectrometry (GC-MS). The highest content of opiate alkaloids in the brain tissue of female animals was found 15 min and in male animals 45 min after treatment. The highest content of opiates was determined in the basal ganglia of the animals of both genders, indicating that this part of brain tissue presents a reliable sample for identifying and assessing contents of opiates after heroin intake.
本研究检测了缴获海洛因中阿片生物碱在实验动物脑区的区域分布，以选择阿片含量最高的部位。对其进行分析应有助于查明海洛因摄入导致的死亡原因。在雄性和雌性Wistar大鼠用缴获的海洛因处理后的不同时间段（5、15、45和120分钟）进行了测试。通过气相色谱-质谱联用（GC-MS）对已知µ-阿片受体浓度较高的脑区（皮层、脑干、杏仁核和基底神经节）中的阿片生物碱（可待因、吗啡、乙酰可待因、6-乙酰吗啡和3,6-二乙酰吗啡）进行了定量测定。在处理后15分钟发现雌性动物脑组织中阿片生物碱含量最高，雄性动物在45分钟时含量最高。在两性动物的基底神经节中均检测到最高含量的阿片类物质，这表明该部分脑组织是识别和评估海洛因摄入后阿片类物质含量的可靠样本。
