Gallego Carolina, Middleton Andrew M, Martínez Nhora, Romero Stefany, Iregui Carlos
Department of Veterinary Pathology, University of Applied and Environmental Sciences, Bogotá, Colombia.
Vet Med Int. 2013;2013:347086. doi: 10.1155/2013/347086. Epub 2013 Mar 11.
The nasal septa of fetal rabbits at 26 days of gestation were harvested by cesarean section of the does while under anesthesia and then exposed to Bordetella bronchiseptica or its lipopolysaccharide (LPS) for periods of 2 and 4 hours. A total of 240 explants were used. The tissues were examined using the Hematoxylin & Eosin technique. Then, semithin sections (0.5 μm) were stained with toluidine blue and examined with indirect immunoperoxidase (IPI) and lectin histochemistry. The most frequent and statistically significant findings were as follows: (1) cell death and increased goblet cell activity when exposed to bacteria and (2) cell death, cytoplasmic vacuolation and infiltration of polymorphonuclear leukocytes when exposed to LPS. The lesions induced by the bacterium were more severe than with LPS alone, except for the cytoplasmic vacuolation in epithelial cells. IPI stained the ciliated border of the epithelium with the bacterium more intensely, while LPS lectin histochemistry preferentially labeled the cytoplasm of goblet cell. These data indicate that B. bronchiseptica and its LPS may have an affinity for specific glycoproteins that would act as adhesion receptors in both locations.
在妊娠26天的胎兔麻醉状态下通过剖宫产获取鼻中隔，然后将其暴露于支气管败血波氏杆菌或其脂多糖（LPS）中2小时和4小时。总共使用了240个外植体。使用苏木精和伊红技术检查组织。然后，将半薄切片（0.5μm）用甲苯胺蓝染色，并用间接免疫过氧化物酶（IPI）和凝集素组织化学检查。最常见且具有统计学意义的结果如下：（1）暴露于细菌时细胞死亡和杯状细胞活性增加；（2）暴露于LPS时细胞死亡、细胞质空泡化和多形核白细胞浸润。除上皮细胞中的细胞质空泡化外，细菌诱导的病变比单独使用LPS更严重。IPI对感染细菌的上皮纤毛边界染色更强烈，而LPS凝集素组织化学优先标记杯状细胞的细胞质。这些数据表明支气管败血波氏杆菌及其LPS可能对特定糖蛋白具有亲和力，这些糖蛋白在两个部位均可作为粘附受体。
