Moorkamp Lars, Nathues Heiko, Spergser Joachim, Tegeler Regina, Grosse Beilage Elisabeth
Field Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, Bakum 49456, Germany.
Vet J. 2008 Feb;175(2):273-5. doi: 10.1016/j.tvjl.2007.01.008. Epub 2007 Mar 6.
The objective of this study was to compare the detection rate of bacterial agents in bronchoalveolar lavage fluid (BALF), taken without visual control, to that in affected lung tissue obtained from the same pig at necropsy. BALF and affected lung tissue were examined for Mycoplasma hyopneumoniae using PCR, and standard cultural methods were used for Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida and Streptococcus suis. All pigs with a history of respiratory symptoms were submitted as live animals for routine diagnostic examination. In each animal the site of lavage, marked by injecting methylene blue, differed from the site of pneumonic lesions. M. hyopneumoniae was detected more frequently in lung tissue than in BALF in cases with moderate or severe lung lesions. The detection rates of M. hyopneumoniae were higher in the BALF of pigs with mild lesions. Cultural examination of BALF was at least as satisfactory as affected lung tissue for detecting B. bronchiseptica, H. parasuis and P. multocida.
本研究的目的是比较在无视觉控制下采集的支气管肺泡灌洗液（BALF）中细菌病原体的检出率与同一头猪尸检时从受影响肺组织中获得的细菌病原体检出率。使用PCR检测BALF和受影响肺组织中的猪肺炎支原体，并采用标准培养方法检测胸膜肺炎放线杆菌、支气管败血波氏杆菌、副猪嗜血杆菌、多杀性巴氏杆菌和猪链球菌。所有有呼吸道症状病史的猪均作为活体动物提交进行常规诊断检查。在每只动物中，通过注射亚甲蓝标记的灌洗部位与肺部病变部位不同。在中度或重度肺部病变病例中，肺组织中猪肺炎支原体的检出频率高于BALF。在轻度病变猪的BALF中，猪肺炎支原体的检出率更高。对于检测支气管败血波氏杆菌、副猪嗜血杆菌和多杀性巴氏杆菌，BALF的培养检查至少与受影响肺组织一样令人满意。
