葡萄糖-6-磷酸脱氢酶缺乏症中的红细胞NADP⁺和NADPH
Red cell NADP+ and NADPH in glucose-6-phosphate dehydrogenase deficiency.
作者信息
Kirkman H N, Gaetani G D, Clemons E H, Mareni C
出版信息
J Clin Invest. 1975 Apr;55(4):875-8. doi: 10.1172/JCI107998.
Abstract
Extraction in the presence of sodium hydroxide and cysteine allows estimates of NADPH and total NADP in human red cells without the erroneously high values of NADP+ obtained with earlier methods. An application of this technique to G6PD-deficient cells reveals that most of the nucleotide is in the oxidized form. In contrast, normal red cells have nearly all of the nucleotide in the reudced form. In addition to providing information concerning the intracellular regulation of the hexose monophosphate shunt, these findings support the concept that G6PD deficiency is a product-deficiency disorder.
摘要
在氢氧化钠和半胱氨酸存在的情况下进行提取，可以对人类红细胞中的还原型辅酶Ⅱ（NADPH）和总辅酶Ⅱ（NADP）进行估算，而不会出现早期方法所得到的错误高值辅酶Ⅱ（NADP⁺）。将该技术应用于葡萄糖-6-磷酸脱氢酶（G6PD）缺乏的细胞时发现，大部分核苷酸处于氧化形式。相比之下，正常红细胞几乎所有的核苷酸都处于还原形式。这些发现除了提供有关己糖磷酸支路细胞内调节的信息外，还支持了葡萄糖-6-磷酸脱氢酶缺乏是一种产物缺乏症的概念。
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