Universidade Luterana do Brasil (ULBRA), Laboratório de Genética Toxicológica, Canoas, RS, Brazil.
J Toxicol Environ Health A. 2013;76(6):381-90. doi: 10.1080/15287394.2012.761947.
Arrabidaea chica Verlot (Bignoniaceae) is an important folk medicine plant native to the Amazon region and used to treat anemia, hemorrhage, inflammation, intestinal colic, hepatitis, and skin affections. Although studies showed its therapeutic properties, little knowledge regarding genotoxic properties of this plant is available. The aim of this study was to determine the potential mutagenic and genotoxic/antigenotoxic effects of an A. chica chloroformic fraction (Ac-CF) obtained from leaves containing bioactive metabolites. The mutagenic effects were evaluated using the Salmonella mutagenicity assay, with TA98, TA97a, TA100, TA102, and TA1535 strains, with and without metabolic activation. In vivo mutagenic and genotoxic/antigenotoxic effects were investigated using the micronucleus (MN) test in bone marrow and alkaline comet assay in blood and liver after administration of 100, 500, or 1000 mg/kg Ac-CF in CF-1 mice by gavage (once a day for 3 d). In vitro antioxidant potential was evaluated using DPPH and xanthine/hypoxanthine assays. Ac-CF was not mutagenic in any of the Salmonella typhimurium strains tested and showed negative responses for mutagenicity and genotoxicity in mice. Further, Ac-CF displayed antigenotoxic effects by decreasing the oxidative DNA damage induced by hydrogen peroxide by greater than 50% in blood and liver. The antioxidant action detected in the in vitro assays demonstrated IC50 of 0.838 mg/ml in the xanthine/hypoxanthine assay and IC50 of 28.17 μg/ml in the DPPH assay. In conclusion, Ac-CF did not induce mutagenic and genotoxic effects and was able to protect DNA against oxidative damage in vivo, suggesting that this fraction may not pose genetic risks, although further toxicology assays are necessary.
阿克拉比德阿夏卡（Bignoniaceae）是一种重要的民间药用植物，原产于亚马逊地区，用于治疗贫血、出血、炎症、肠痉挛、肝炎和皮肤病。尽管研究表明它具有治疗特性，但对这种植物的遗传毒性知之甚少。本研究旨在确定从含有生物活性代谢物的叶子中获得的阿克拉比德阿夏卡氯仿提取物（Ac-CF）的潜在致突变和遗传毒性/抗原毒性作用。使用沙门氏菌致突变试验，用 TA98、TA97a、TA100、TA102 和 TA1535 菌株，在有或没有代谢激活的情况下，评估致突变作用。体内致突变和遗传毒性/抗原毒性作用通过灌胃（每天一次，连续 3 天）给予 CF-1 小鼠 100、500 或 1000mg/kg Ac-CF 后，在骨髓中的微核（MN）试验和血液和肝脏中的碱性彗星试验中进行研究。使用 DPPH 和黄嘌呤/次黄嘌呤测定法评估体外抗氧化潜力。在测试的任何鼠伤寒沙门氏菌菌株中，Ac-CF 均无致突变性，并且在小鼠中未显示出致突变性和遗传毒性。此外，Ac-CF 通过降低血液和肝脏中过氧化氢诱导的氧化 DNA 损伤，显示出大于 50%的抗原毒性作用。在体外试验中检测到的抗氧化作用在黄嘌呤/次黄嘌呤试验中显示出 IC50 为 0.838mg/ml，在 DPPH 试验中显示出 IC50 为 28.17μg/ml。总之，Ac-CF 没有诱导致突变和遗传毒性作用，并且能够在体内保护 DNA 免受氧化损伤，这表明该馏分可能不会造成遗传风险，尽管需要进一步的毒理学试验。
