Eiger S, Mills K T, Bern H A
Department of Integrative Biology, University of California, Berkeley 94720.
J Steroid Biochem. 1990 Apr;35(5):617-21. doi: 10.1016/0022-4731(90)90207-9.
Steroid binding in both the vaginal epithelium and the vaginal fibromuscular wall (FMW) was compared in control and neonatally estrogen-treated mice. Neonatal treatment with a low dose of the estrogen diethylstilbestrol (DES) had no significant effect on adult estrogen binding within the assayed vaginal compartments; however, this treatment caused a 2-fold increase in the level of cytosolic progestin binding in the vaginal FMW over that in vehicle-treated mice. This low neonatal dose did not affect the level of progestin binding in the vaginal epithelium. In contrast, neonatal treatment with a larger dose of DES caused marked increases in cytosolic progestin binding, decreases in cytosolic estrogen binding, and increases in nuclear estrogen binding within the FMW. Furthermore, as a result of the changes in specific binding induced by the neonatal DES treatment, the degree of the estrogen binding within in each tissue shifted from a predominantly cytosolic site to a nuclear one.
在对照小鼠和新生期接受雌激素处理的小鼠中，对阴道上皮和阴道纤维肌壁（FMW）中的类固醇结合情况进行了比较。用低剂量雌激素己烯雌酚（DES）进行新生期处理，对所检测的阴道隔室内成年期雌激素结合没有显著影响；然而，与用赋形剂处理的小鼠相比，这种处理使阴道FMW中胞质孕激素结合水平增加了2倍。这种低剂量的新生期处理并不影响阴道上皮中孕激素结合水平。相比之下，用较大剂量DES进行新生期处理会导致FMW中胞质孕激素结合显著增加、胞质雌激素结合减少以及核雌激素结合增加。此外，由于新生期DES处理引起的特异性结合变化，每个组织内雌激素结合的程度从主要位于胞质部位转变为位于核部位。
