Department of Bionano Engineering, Hanyang University, Sa-1-dong 1271, Ansan 426-791, Republic of Korea.
Biosens Bioelectron. 2013 Sep 15;47:62-7. doi: 10.1016/j.bios.2013.03.003. Epub 2013 Mar 14.
This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA15) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA29)-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease.
本文报道了一种基于表面增强拉曼散射（SERS）的磁性适体传感器高灵敏度检测凝血酶的方法。磁珠和金纳米粒子（Au NPs）分别用作支撑基底和传感探针。为此，将 15 -mer 凝血酶结合适体（TBA15）固定在磁珠表面，然后依次加入凝血酶抗原和 29-mer 凝血酶结合适体（TBA29）-共轭 Au NPs 以形成三明治适体复合物。通过监测拉曼报告分子的特征 SERS 信号强度变化进行定量分析。由于所有反应都在溶液中进行，因此这种基于 SERS 的免疫分析技术可以解决固体质子扩散受限的动力学问题。基于 SERS 的适体传感器测定的凝血酶的检测限（LOD）估计为 0.27pM。该方法有望成为血栓性疾病诊断的良好临床工具。
