Yu Qian, Wu Yixuan, Kang Taejoon, Choo Jaebum
Department of Chemistry, Chung-Ang University Seoul South Korea.
Bionanotechnology Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon South Korea.
Bull Korean Chem Soc. 2021 Dec;42(12):1699-1705. doi: 10.1002/bkcs.12418. Epub 2021 Oct 14.
We developed a novel surface-enhanced Raman scattering (SERS)-based SARS-CoV-2 assay platform using hollow Au nanostars to realize high-sensitivity diagnosis of SARS-CoV-2. The assay was performed using SARS-CoV-2 lysate as the target in a wide dynamic range with virus concentrations ranging from 0 to 10 PFU/ml and has a limit of detection (LOD) of 5.1 PFU/ml. This LOD value shows 100 times and 10 times better sensitivity compared to the LODs measured on the same sample using a commercially available rapid kit and enzyme-linked immunosorbent assay, respectively. Therefore, we believe that this SERS-based SARS-CoV-2 assay platform has high diagnostic accuracy for early or asymptomatic infected patients with low virus concentrations. Furthermore, the probability of a false-negative diagnosis is likely to be very low.
我们开发了一种基于新型表面增强拉曼散射（SERS）的新型冠状病毒检测平台，该平台使用空心金纳米星来实现对新型冠状病毒的高灵敏度诊断。该检测方法以新型冠状病毒裂解液为靶标，在0至10 PFU/ml的病毒浓度宽动态范围内进行，检测限（LOD）为5.1 PFU/ml。与使用市售快速检测试剂盒和酶联免疫吸附测定法在同一样品上测得的检测限相比，该检测限分别显示出高100倍和10倍的灵敏度。因此，我们认为这种基于SERS的新型冠状病毒检测平台对于病毒浓度较低的早期或无症状感染患者具有较高的诊断准确性。此外，假阴性诊断的可能性可能非常低。
