克隆仓鼠抗鼠 CD79B 抗体序列和鉴定新的仓鼠免疫球蛋白 lambda 恒定 IGLC 基因区域。
Cloning of a hamster anti-mouse CD79B antibody sequences and identification of a new hamster immunoglobulin lambda constant IGLC gene region.
机构信息
Department of Medicine, Section of Rheumatology, Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, IL 60637, USA.
出版信息
Immunogenetics. 2013 Jun;65(6):473-8. doi: 10.1007/s00251-013-0698-5. Epub 2013 Apr 5.
Abstract
Anti-CD79 antibodies have been effective at targeting B cell lymphoma cells and depleting B cells in animal models. In order to engineer recombinant antibodies with additional effector functions in mice, we cloned and sequenced the full-length cDNAs of the heavy and light chain of a hamster anti-mouse CD79B antibody. Although hamster antibodies represent a unique source of monoclonal antibodies against mouse, rat, and human antigens, sequence information of hamster immunoglobulins (IG) is sparse. Here, we report a new hamster (Cricetulus migratorius) IG lambda constant (IGLC) gene region that is most homologous to mouse IGLC2 and IGLC3.
摘要
抗 CD79 抗体在靶向 B 细胞淋巴瘤细胞和耗尽动物模型中的 B 细胞方面非常有效。为了在小鼠中构建具有额外效应功能的重组抗体，我们克隆并测序了仓鼠抗小鼠 CD79B 抗体的重链和轻链全长 cDNA。尽管仓鼠抗体代表了针对小鼠、大鼠和人抗原的独特单克隆抗体来源，但仓鼠免疫球蛋白 (IG) 的序列信息却很少。在这里，我们报告了一个新的仓鼠 (Cricetulus migratorius) IG lambda 恒定区 (IGLC)，它与小鼠 IGLC2 和 IGLC3 最为同源。
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