Xu Hao, Wang Zhanxiang, Zhang Shaolin, Tan Guowei, Zhu Hongwei
Department of Neurosurgery, First Affiliate Hospital of Xiamen University, Xiamen, Fujian Province, People's Republic of China.
J Neurosurg Pediatr. 2013 Jun;11(6):692-6. doi: 10.3171/2013.2.PEDS12324. Epub 2013 Apr 5.
Fibrosis along the route of CSF flow is indicated by the development of hydrocephalus. The changes of fibrosis index might reflect the level of hydrocephalus and even become a diagnostic index of hydrocephalus. The object of this study was to analyze the levels of procollagen Type I C-terminal propeptide (PICP), procollagen Type III N-terminal propeptide (PIIINP), hyaluronic acid (HA), and laminin (LN) and their significance in the CSF of communicating hydrocephalus rat models.
Thirty adult Sprague-Dawley rats were randomly divided into 3 groups: hydrocephalus group (20 rats) with intraventricular kaolin injections, sham control group (5 rats) with saline injections, and normal group (5 rats) without any processing. The levels of PICP, PIIINP, HA, and LN in the CSF were detected using ELISA.
Levels of PICP, PIIINP, HA, and LN in the hydrocephalus group were significantly higher than those in the saline control group (p < 0.05). It was revealed by correlation analysis that the increase was positively correlated with the severity of ventricular dilation.
Results indicated that PICP, PIIINP, HA, and LN continue to rise dramatically in experimental hydrocephalus and may serve as the diagnostic index of hydrocephalus.
脑积水的发生提示脑脊液（CSF）流动路径上存在纤维化。纤维化指标的变化可能反映脑积水的程度，甚至可成为脑积水的诊断指标。本研究旨在分析交通性脑积水大鼠模型脑脊液中Ⅰ型前胶原C端前肽（PICP）、Ⅲ型前胶原N端前肽（PIIINP）、透明质酸（HA）和层粘连蛋白（LN）的水平及其意义。
将30只成年Sprague-Dawley大鼠随机分为3组：脑室注射高岭土的脑积水组（20只大鼠）、注射生理盐水的假手术对照组（5只大鼠）和未作任何处理的正常组（5只大鼠）。采用酶联免疫吸附测定法（ELISA）检测脑脊液中PICP、PIIINP、HA和LN的水平。
脑积水组PICP、PIIINP、HA和LN的水平显著高于生理盐水对照组（p < 0.05）。相关性分析显示，这些指标的升高与脑室扩张的严重程度呈正相关。
结果表明，在实验性脑积水中PICP、PIIINP、HA和LN持续显著升高，可能作为脑积水的诊断指标。
