Søndergaard K, Heickendorff L, Risteli L, Risteli J, Zachariae H, Stengaard-Pedersen K, Deleuran B
Department of Rheumatology, Aarhus University Hospital, Denmark.
Br J Dermatol. 1997 Jan;136(1):47-53.
The aminoterminal propeptide of type III procollagen (PIIINP) and the carboxyterminal propeptide of type I procollagen (PICP) and hyaluronan (HA) were measured in plasma and suction blister fluid from 13 systemic sclerosis patients and 11 healthy volunteers. Suction blisters and skin biopsies were from the transition zone between normal skin and scleroderma, and uninvolved abdominal skin of patients. The median value of suction blister PIIINP from the transition zone was 38% higher than suction blister PIIINP from uninvolved skin. PIIINP was localized to the dermis by immunohistochemical techniques. PICP and HA levels in blisters from the transition zone were 87% and 53%, respectively, above the levels measured in uninvolved skin. Furthermore, PICP and HA blister levels from the transition zone were 67% and 63%, respectively, higher than the levels measured in healthy volunteers. In plasma from scleroderma patients levels of PIIINP and HA were 38% and 127% higher, respectively, than in plasma of healthy volunteers. The plasma PICP level was not significantly higher in scleroderma patients. Finally, PICP, PIIINP and HA levels were several times higher in suction blister fluid than in plasma. The data indicate that a fibrogenetic process takes place in the transition zone of scleroderma. The method may be used to monitor the progression of scleroderma skin lesions in vivo.
在13例系统性硬化症患者和11名健康志愿者的血浆及抽吸水疱液中，检测了III型前胶原氨基端前肽（PIIINP）、I型前胶原羧基端前肽（PICP）和透明质酸（HA）。抽吸水疱和皮肤活检样本取自硬皮病患者正常皮肤与硬皮病病变皮肤的过渡区以及未受累的腹部皮肤。过渡区抽吸水疱液中PIIINP的中位数比未受累皮肤抽吸水疱液中的PIIINP高38%。通过免疫组织化学技术将PIIINP定位于真皮。过渡区水疱中PICP和HA的水平分别比未受累皮肤中测得的水平高87%和53%。此外，过渡区水疱中PICP和HA的水平分别比健康志愿者中测得的水平高67%和63%。在系统性硬化症患者的血浆中，PIIINP和HA的水平分别比健康志愿者血浆中的水平高38%和127%。系统性硬化症患者血浆中的PICP水平没有显著升高。最后，抽吸水疱液中PICP、PIIINP和HA的水平比血浆中的水平高几倍。数据表明在硬皮病的过渡区发生了纤维化过程。该方法可用于在体内监测硬皮病皮肤病变的进展。
