Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2013;6:237-65. doi: 10.1146/annurev-anchem-062012-092609. Epub 2013 Apr 3.
The field of glycomics has recently advanced in response to the urgent need for structural characterization and quantification of complex carbohydrates in biologically and medically important applications. The recent success of analytical glycobiology at high sensitivity reflects numerous advances in biomolecular mass spectrometry and its instrumentation, capillary and microchip separation techniques, and microchemical manipulations of carbohydrate reactivity. The multimethodological approach appears to be necessary to gain an in-depth understanding of very complex glycomes in different biological systems.
糖组学领域最近取得了进展,以满足在具有生物学和医学重要性的应用中对复杂碳水化合物进行结构描述和定量的迫切需求。分析糖生物学在高灵敏度方面的最新成功反映了生物分子质谱及其仪器、毛细管和微芯片分离技术以及碳水化合物反应性的微化学操作方面的许多进展。似乎需要采用多方法学方法才能深入了解不同生物系统中非常复杂的糖组。