Cox N A, Bailey J S, Del Corral F, Shotts E B
USDA/ARS-Richard B. Russell Agricultural Research Center, Athens, Georgia 30613.
Poult Sci. 1990 Apr;69(4):686-93. doi: 10.3382/ps.0690686.
Yersinia enterocolitica (Serotypes 0:3 or 0:8), Yersinia frederiksenii, Yersinia kristensenii, or Yersinia intermedia along with 10(8) cells of each of three extraneous organisms (Escherichia coli, Enterobacter aerogenes, Pseudomonas fragi), all commonly found on market poultry, were inoculated into five enrichment media followed by streaking onto 11 plating media to determine the most-efficacious combinations for future surveys or assessment studies. For Yersinia enterocolitica (0:8), infrequent recoveries were made using yeast extract-rosebengal-bile oxalate sorbose broth and phosphate-buffered saline (4 C) followed by plating onto pectin, DNase-Tween 80 (polyoxyethylene sorbitan monooleate)-sorbitol, MacConkey-Tween 80, or cefsulodin-irgasan-novobiocin (CIN) agars. With Y. enterocolitica (0:3), recoveries were most frequently made using phosphate-buffered saline, sorbitol bile (incubated for 17 days) and yeast extract-rosebengal-bile oxalate sorbose broth followed by plating onto pectin, CIN, bismuth sulfite (Difco Laboratories, Detroit, MI), or modified Rimler-Shotts agar. For Y. frederiksenii, Y. kristensenii, and Y. intermedia, incubation in sorbitol bile for 17 days or in yeast extract-rosebengal-bile oxalate sorbose broth, followed by plating onto CIN, pectin, DNase-Tween/80-sorbitol, cellobiose-arginine-lysine agar, or MacConkey-Tween 80 agar yielded the most-frequent recoveries. Overall, the CIN and pectin agars performed best for the recovery of the Yersinia bacterium; the modified selenite broth and the bismuth-sulfite plating agars were unsatisfactory in the present study.
小肠结肠炎耶尔森菌（血清型0:3或0:8）、费氏耶尔森菌、克氏耶尔森菌或中间耶尔森菌，以及三种常见于市售家禽的外源菌（大肠杆菌、产气肠杆菌、脆弱假单胞菌）各10⁸个细胞，被接种到五种增菌培养基中，随后划线接种到11种平板培养基上，以确定未来调查或评估研究中最有效的组合。对于小肠结肠炎耶尔森菌（0:8），使用酵母提取物-孟加拉红-草酸胆盐山梨醇肉汤和磷酸盐缓冲盐水（4℃）进行增菌，随后接种到果胶、脱氧核糖核酸酶-吐温80（聚氧乙烯山梨醇单油酸酯）-山梨醇、麦康凯-吐温80或头孢磺啶-伊红-新生霉素（CIN）琼脂平板上，回收率较低。对于小肠结肠炎耶尔森菌（0:3），使用磷酸盐缓冲盐水、山梨醇胆汁（培养17天）和酵母提取物-孟加拉红-草酸胆盐山梨醇肉汤进行增菌，随后接种到果胶、CIN、亚硫酸铋（迪夫科实验室，底特律，密歇根州）或改良的林勒-肖茨琼脂平板上，回收率最高。对于费氏耶尔森菌、克氏耶尔森菌和中间耶尔森菌，在山梨醇胆汁中培养17天或在酵母提取物-孟加拉红-草酸胆盐山梨醇肉汤中培养，随后接种到CIN、果胶、脱氧核糖核酸酶-吐温/80-山梨醇、纤维二糖-精氨酸-赖氨酸琼脂或麦康凯-吐温80琼脂平板上，回收率最高。总体而言，CIN和果胶琼脂对耶尔森菌的回收率表现最佳；在本研究中，改良亚硒酸盐肉汤和亚硫酸铋平板琼脂效果不佳。
