Cox N A, Del Corral F, Bailey J S, Shotts E B, Papa C M
Russell Agricultural Research Center, Athens, Georgia 30613.
Poult Sci. 1990 Mar;69(3):482-5. doi: 10.3382/ps.0690482.
Sixty ready-to-cook broiler carcasses obtained from several local supermarkets were tested for the presence of Yersinia enterocolitica and other Yersinia species. In the present study, the authors used two enrichment broths, yeast-extract/rosebengal-bile oxalate sorbose (YER-BOS) and phosphate-buffered saline with a postenrichment KOH treatment (PBS-KOH), and two plating media, cefsulodin-irgasan-novobiocin (CIN) and pectin agar. Yersinia organisms were found on 34 of 60 carcasses (56.7%) and Y. enterocolitica, on 16 of 60 carcasses (26.7%). There was no significant difference between CIN and pectin agar; however, PBS-KOH yielded a significantly higher (P less than or equal to .05) detection rate than YER-BOS, regardless of the plating media used. In addition to Y. enterocolitica, Y. frederiksenii and Y. intermedia were also isolated from the market broilers. None of the Y. enterocolitica isolates were found to be presumptively virulent.
从当地几家超市获取了60个待烹饪的肉鸡 carcasses，检测其中小肠结肠炎耶尔森菌和其他耶尔森菌属的存在情况。在本研究中，作者使用了两种增菌肉汤，即酵母提取物/孟加拉红-草酸盐山梨醇（YER-BOS）和经过增菌后氢氧化钾处理的磷酸盐缓冲盐水（PBS-KOH），以及两种平板培养基，即头孢磺啶-伊红-新生霉素（CIN）和果胶琼脂。在60个 carcasses 中的34个（56.7%）上发现了耶尔森菌属微生物，在60个 carcasses 中的16个（26.7%）上发现了小肠结肠炎耶尔森菌。CIN和果胶琼脂之间没有显著差异；然而，无论使用哪种平板培养基，PBS-KOH的检出率都显著高于YER-BOS（P小于或等于0.05）。除小肠结肠炎耶尔森菌外，还从市售肉鸡中分离出了费氏耶尔森菌和中间耶尔森菌。未发现任何小肠结肠炎耶尔森菌分离株具有推定的致病性。
