Walker S J, Gilmour A
J Appl Bacteriol. 1986 Mar;60(3):175-83. doi: 10.1111/j.1365-2672.1986.tb01071.x.
A number of plating and enrichment media proposed for the isolation of Yersinia enterocolitica from foodstuffs were examined for their ability to recover the type strains of Y. enterocolitica sensu stricto, Y. intermedia, Y. frederiksenii and Y. kristensenii. Nine selective plating media were evaluated for the quantitative recovery of the type strains in pure culture, and their inhibition of other organisms typical of both milk and enteric microfloras. Cefsulodin-irgasan-novobiocin (CIN) agar, incubated for 48 h at 25 degrees C, allowed a high recovery of all the Yersinia spp. and was the most selective medium. The same four type strains were added to UHT milk that had been previously inoculated with bacteria to simulate either freshly drawn or cold stored milk microfloras. Twenty-six enrichment procedures (including cold enrichment, selective enrichment at higher temperatures, two-step procedures and a post-enrichment alkali treatment) were assessed for the efficiency of recovery of the Yersinia spp. Pre-enrichment in trypticase-soy broth (TSB) for 24 h at 22 degrees C followed by selective enrichment in bile-oxalate-sorbose (BOS) medium for 5 d at 22 degrees C and plating on CIN agar (48 h at 25 degrees C) allowed the greatest increase in the numbers of Yersinia spp. and maximum inhibition of the competing microflora.
对一些为从食品中分离小肠结肠炎耶尔森菌而提出的平板计数和增菌培养基，检测了其复苏小肠结肠炎耶尔森菌狭义生物群、中间型耶尔森菌、费氏耶尔森菌和克里斯滕森耶尔森菌模式菌株的能力。评估了9种选择性平板培养基对模式菌株纯培养物的定量复苏能力，以及它们对牛奶和肠道微生物群中典型的其他微生物的抑制作用。头孢磺啶-伊红-新生霉素（CIN）琼脂在25℃下培养48小时，能使所有耶尔森菌属菌株得到较高的复苏率，是最具选择性的培养基。将同样的4种模式菌株添加到先前已接种细菌的超高温灭菌牛奶中，以模拟新鲜采集或冷藏的牛奶微生物群。评估了26种增菌程序（包括冷增菌、较高温度下的选择性增菌、两步程序和增菌后碱处理）对耶尔森菌属菌株的复苏效率。在胰蛋白酶大豆肉汤（TSB）中于22℃预增菌24小时，然后在胆盐-草酸盐-山梨糖（BOS）培养基中于22℃选择性增菌5天，并接种到CIN琼脂上（25℃培养48小时），能使耶尔森菌属菌株数量增加最多，并最大程度地抑制竞争性微生物群。
