INFIQC, Departamento de Físico Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina.
Colloids Surf B Biointerfaces. 2013 Aug 1;108:329-36. doi: 10.1016/j.colsurfb.2013.02.028. Epub 2013 Mar 14.
We report for the first time the use of double stranded calf-thymus DNA (dsDNA) to successfully disperse bamboo-like multi-walled carbon nanotubes (bCNT). The dispersion and the modified electrodes were studied by different spectroscopic, microscopic and electrochemical techniques. The drastic treatment for dispersing the bCNT (45min sonication in a 50% (v/v) ethanol:water solution), produces a partial denaturation and a decrease in the length of dsDNA that facilitates the dispersion of CNT and makes possible an efficient electron transfer of guanine residues to the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of glassy carbon electrodes (GCE) modified with bCNT-dsDNA dispersion is also reported. The electron transfer of redox probes and guanine residues was more efficient at GCE modified with bCNT dispersed in dsDNA than at GCE modified with hollow CNT (hCNT) dispersed in dsDNA, demonstrating the importance of the presence of bCNT.
我们首次报道了使用双链小牛胸腺 DNA（dsDNA）成功分散竹状多壁碳纳米管（bCNT）。通过不同的光谱、显微镜和电化学技术研究了分散和修饰电极。剧烈处理分散 bCNT（在 50%（v/v）乙醇：水溶液中超声 45 分钟）会导致 dsDNA 部分变性和长度减小，从而促进 CNT 的分散，并使鸟嘌呤残基向电极的有效电子转移成为可能。还报告了对不同实验条件对分散效率和用 bCNT-dsDNA 分散体修饰的玻璃碳电极（GCE）性能的影响进行了批判性分析。在 GCE 上修饰 bCNT 分散在 dsDNA 中比在 GCE 上修饰空心 CNT（hCNT）分散在 dsDNA 中，氧化还原探针和鸟嘌呤残基的电子转移效率更高，这证明了 bCNT 的存在很重要。
