Erdem Arzum, Papakonstantinou Pagona, Murphy Hayley
Analytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. arzum.
Anal Chem. 2006 Sep 15;78(18):6656-9. doi: 10.1021/ac060202z.
The performance of glassy carbon (GCE) and graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (CNTs) are compared, based on the direct electrochemical detection of nucleic acids. This is accomplished by monitoring the differential pulse voltammetry changes of the guanine signal. CNT-modified PGE compares favorably to that of the commonly used CNT-modified GCE owing to the intrinsic improved performance of the supporting PGE. The better intrinsic characteristics of the PGE are related to its composite structure and higher level of porosity compared to GCE. The performance characteristics of the direct DNA hybridization on the disposable CNT-modified PGE are studied in terms of optimum analytical conditions such as probe concentration, target concentration, hybridization time, and selectivity. The new DNA biosensor described here has shown some important advantages such being inexpensive, sensitive, selective, and able to generate reproducible results using a simple and direct electrochemical protocol.
基于核酸的直接电化学检测，比较了用多壁碳纳米管（CNT）修饰的玻碳电极（GCE）和石墨铅笔电极（PGE）的性能。这是通过监测鸟嘌呤信号的差分脉冲伏安变化来实现的。由于支撑PGE的固有性能提高，CNT修饰的PGE与常用的CNT修饰的GCE相比具有优势。与GCE相比，PGE更好的固有特性与其复合结构和更高的孔隙率有关。从探针浓度、靶标浓度、杂交时间和选择性等最佳分析条件方面研究了一次性CNT修饰PGE上直接DNA杂交的性能特征。这里描述的新型DNA生物传感器显示出一些重要优点，如价格便宜、灵敏、有选择性，并且能够使用简单直接的电化学方案产生可重复的结果。
