口腔鳞状细胞癌的 miRNA 表达谱。
miRNA expression profiles of oral squamous cell carcinomas.
机构信息
Institute of Public Health, Faculty of Medicine, Clinic Center University of Pécs, Pécs, Hungary.
出版信息
Anticancer Res. 2013 Apr;33(4):1511-7.
BACKGROUND
Oral squamous cell carcinoma (OSCC) development involves complex machinery of genomic and epigenetic regulations including microRNA (miRNA) expression changes. We aimed to analyze the expression patterns of a set of miRNAs in human TNM stage I and II OSCC samples in an autologous normal mucosa-controlled experimental design.
MATERIALS AND METHODS
Forty samples of OSCC and 40 matched normal tissues were evaluated for miR-21, miR-155, miR-191, miR-146a, miR-221 and miR-222 expression in a LightCycler 480(PCR) system.
RESULTS
Our results showed significant overexpression of miR-21, miR-155, miR-191 and miR-221 in paired-sample t-test and the sensitivity/specificity of tests were over 90% in the case of miR-21 and miR-155 on the receiver operating characteristic curve (ROC) analysis.
CONCLUSION
Our results underline the role of miR-21 in OSCC and support the possible causal role of miR-155 and miR-221 in oral carcinogenesis. The overexpression of miR-191 is a novel finding in squamous cell carcinoma.
背景
口腔鳞状细胞癌（OSCC）的发展涉及基因组和表观遗传调控的复杂机制，包括 miRNA（miRNA）表达变化。我们旨在分析一组 miRNA 在人类 TNM 分期 I 和 II OSCC 样本中的表达模式，采用自体正常黏膜对照的实验设计。
材料和方法
在 LightCycler 480（PCR）系统中评估了 40 例 OSCC 和 40 例配对正常组织中 miR-21、miR-155、miR-191、miR-146a、miR-221 和 miR-222 的表达。
结果
我们的结果显示，miR-21、miR-155、miR-191 和 miR-221 在配对样本 t 检验中呈显著过表达，miR-21 和 miR-155 的测试灵敏度/特异性在 ROC 分析中超过 90%。
结论
我们的结果强调了 miR-21 在 OSCC 中的作用，并支持 miR-155 和 miR-221 在口腔癌变中的可能因果作用。miR-191 的过表达是鳞状细胞癌中的一个新发现。
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