Institute of Public Health, Faculty of Medicine, Clinic Center University of Pécs, Pécs, Hungary.
Anticancer Res. 2013 Apr;33(4):1511-7.
Oral squamous cell carcinoma (OSCC) development involves complex machinery of genomic and epigenetic regulations including microRNA (miRNA) expression changes. We aimed to analyze the expression patterns of a set of miRNAs in human TNM stage I and II OSCC samples in an autologous normal mucosa-controlled experimental design.
Forty samples of OSCC and 40 matched normal tissues were evaluated for miR-21, miR-155, miR-191, miR-146a, miR-221 and miR-222 expression in a LightCycler 480(PCR) system.
Our results showed significant overexpression of miR-21, miR-155, miR-191 and miR-221 in paired-sample t-test and the sensitivity/specificity of tests were over 90% in the case of miR-21 and miR-155 on the receiver operating characteristic curve (ROC) analysis.
Our results underline the role of miR-21 in OSCC and support the possible causal role of miR-155 and miR-221 in oral carcinogenesis. The overexpression of miR-191 is a novel finding in squamous cell carcinoma.
口腔鳞状细胞癌（OSCC）的发展涉及基因组和表观遗传调控的复杂机制，包括 miRNA（miRNA）表达变化。我们旨在分析一组 miRNA 在人类 TNM 分期 I 和 II OSCC 样本中的表达模式，采用自体正常黏膜对照的实验设计。
在 LightCycler 480（PCR）系统中评估了 40 例 OSCC 和 40 例配对正常组织中 miR-21、miR-155、miR-191、miR-146a、miR-221 和 miR-222 的表达。
我们的结果显示，miR-21、miR-155、miR-191 和 miR-221 在配对样本 t 检验中呈显著过表达，miR-21 和 miR-155 的测试灵敏度/特异性在 ROC 分析中超过 90%。
我们的结果强调了 miR-21 在 OSCC 中的作用，并支持 miR-155 和 miR-221 在口腔癌变中的可能因果作用。miR-191 的过表达是鳞状细胞癌中的一个新发现。
