Department of Biomedical Engineering & Materials, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan 330-090, South Korea.
J Biomater Sci Polym Ed. 2013;24(5):520-38. doi: 10.1080/09205063.2012.697696. Epub 2012 Aug 13.
In order to augment bone formation, a new biodegradable scaffold system was fabricated using different ratios of hydroxyapatite (HAp) blended with synthetic polymer polycaprolactone (PCL) and natural polymer gelatin (GE) followed by electrospinning method. Three different concentrations of HAp were used in PCL/GE to obtain a blend of 10, 30, and 50% (w/v) HAp-PCL/GE. These HAp-loaded PCL/GE blends were then compared with PCL/GE blends by different mechanical and biological in vitro and in vivo studies to understand the applicability of the system. Scanning electron microscopy, X-ray diffraction analysis, and tensile strength measurement were done to obtain physical properties. Fifty Percent HAp-PCL/GE blends possessed the highest mechanical strength. In vitro cytotoxicity and proliferation of osteoblast cells on the PCL/GE and HAp-PCL/GE scaffolds were examined and shown that addition of HAp in PCL/GE was beneficial by increasing cell viability (>85%) proliferation and cell-surface attachment. Expression of collagen and osteopontin was also found higher in 50% HAp-PCL/GE blends than the others. On the other hand, in vivo bone formation was examined using rat models and increased bone formation was observed in 50% HAp-PCL/GE blends within 6 weeks. Based on the combined results of this study, HAp-PCL/GE membranes were found to hold great promise for use in tissue engineering applications, especially in bone tissue engineering.
为了增强骨形成，我们使用不同比例的羟基磷灰石（HAp）与合成聚合物聚己内酯（PCL）和天然聚合物明胶（GE）混合，然后通过静电纺丝法制备了一种新型可生物降解的支架系统。在 PCL/GE 中使用了三种不同浓度的 HAp，以获得 10、30 和 50%（w/v）HAp-PCL/GE 的混合物。然后通过不同的机械和体外及体内生物学研究比较了这些载有 HAp 的 PCL/GE 共混物，以了解该系统的适用性。进行了扫描电子显微镜、X 射线衍射分析和拉伸强度测量以获得物理性能。50%HAp-PCL/GE 共混物具有最高的机械强度。对 PCL/GE 和 HAp-PCL/GE 支架上成骨细胞的体外细胞毒性和增殖进行了检查，并表明在 PCL/GE 中添加 HAp 通过提高细胞活力（>85%）增殖和细胞表面附着是有益的。还发现 50%HAp-PCL/GE 共混物中胶原蛋白和骨桥蛋白的表达更高。另一方面，使用大鼠模型检查了体内骨形成，并且在 50%HAp-PCL/GE 共混物中观察到 6 周内骨形成增加。基于这项研究的综合结果，HAp-PCL/GE 膜有望在组织工程应用中得到应用，特别是在骨组织工程中。
