兔角膜后表面伤口的内皮修复。培养基中胎牛血清的影响。

Endothelial repair of posterior corneal wounds in rabbits. Influence of foetal calf serum in the culture medium.

作者信息

Medin H, Davanger M

机构信息

University Eye Department, Rikshospitalet, University of Oslo, Norway.

出版信息

Acta Ophthalmol (Copenh). 1990 Apr;68(2):195-9. doi: 10.1111/j.1755-3768.1990.tb01903.x.

DOI:10.1111/j.1755-3768.1990.tb01903.x

PMID:2356708

Abstract

UNLABELLED

The endothelial covering of experimental wounds on the posterior corneal surface has been studied in organ culture experiments. Defects involving the Descemet's membrane were produced, in addition to pure endothelial defects. The influence of foetal calf serum (FCS) on the healing process was examined. Wounds including the Descemet's membrane, about 1-2 X 3 mm, were not covered by cells after 12 days in FCS free medium. Such wounds were covered by endothelium after 6 days when cultivated in medium containing 10% FCS, and partly covered in 1% FCS. Pure endothelial defects, 4 mm in diameter, were cell-covered within 3-4 days irrespective of the presence of FCS.

CONCLUSION

the presence of FCS is required for the healing of defects involving the Descemet's membrane, and not required for the healing of pure endothelial defects.

摘要

未标记

在器官培养实验中对角膜后表面实验性伤口的内皮覆盖情况进行了研究。除了单纯的内皮缺陷外，还制造了涉及后弹力层的缺陷。研究了胎牛血清（FCS）对愈合过程的影响。在不含FCS的培养基中培养12天后，包括后弹力层的约1 - 2×3毫米伤口未被细胞覆盖。当在含10% FCS的培养基中培养时，此类伤口在6天后被内皮覆盖，在含1% FCS的培养基中部分被覆盖。直径4毫米的单纯内皮缺陷，无论是否存在FCS，在3 - 4天内均被细胞覆盖。