College of Agronomy and Biotechnology, Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2013 May;136:481-7. doi: 10.1016/j.biortech.2013.03.015. Epub 2013 Mar 13.
The present study investigated the degradation of un-pretreated wheat straw, corn stalk, and rice straw by a lignocellulose-degrading microbial consortium XDC-2. Following six days of cultivation, exocellular xylanase activities were 414.9, 491.9, and 335 U/mL, respectively. After 12 days, the rice straw had lost 39.71% of its weight, hemicellulose and cellulose losses of 78.27% and 14.08%, respectively. The total amount of volatile products reached a maximum on day six for rice straw degradation. The four major types of volatile products were acetic acid, propionic acid, butanoic acid, and glycerin, all of which would be suitable substrates for conversion to methanol by anaerobic digestion. According to PCR-DGGE analysis, XDC-2 remained stable during the degradation process of untreated lignocellulosic biomass. These results demonstrate the potential for further development and application of XDC-2; it is capable of degrading un-pretreated lignocellulosic materials, and has a low cost of operation.
本研究利用木质纤维素降解微生物菌剂 XDC-2 对未经预处理的小麦秸秆、玉米秸秆和水稻秸秆进行降解。培养 6 天后，胞外木聚糖酶活性分别为 414.9、491.9 和 335 U/mL。12 天后，水稻秸秆的失重率达到 39.71%，半纤维素和纤维素的损失率分别达到 78.27%和 14.08%。在水稻秸秆降解过程中，挥发性产物总量在第 6 天达到最大值。四种主要挥发性产物为乙酸、丙酸、丁酸和甘油，均为厌氧消化转化为甲醇的适宜底物。根据 PCR-DGGE 分析，XDC-2 在未预处理的木质纤维素生物量降解过程中保持稳定。这些结果表明 XDC-2 具有进一步开发和应用的潜力；它能够降解未经预处理的木质纤维素材料，且具有较低的运营成本。
