College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
Bioresour Technol. 2011 Oct;102(19):9321-4. doi: 10.1016/j.biortech.2011.07.065. Epub 2011 Jul 24.
A microbial consortium, designated WCS-6, was established by successive subcultivation in the presence of rice straw under static conditions. The degradation efficiencies of WSC-6 for 0.5 g filter paper, cotton and rice straw after 3 days of cultivation were 99.0±0.7%, 76.9±1.5% and 81.3±0.8%, respectively as determined by gravimetrical methods. Nine bacterial isolates were obtained from WCS-6 plated under aerobic conditions, and sequencing of their 16S rDNA indicated that these bacteria were related to Bacillus thermoamylovorans BTa, Paenibacillus barengoltzii SAFN-016, Proteobacterium S072, Pseudoxanthomonas taiwanensis CB-226, Rhizobiaceae str. M100, Bacillus sp. E53-10, Beta proteobacterium HMD444, Petrobacter succinimandens 4BON, and Tepidiphilus margaritifer N2-214. DGGE (denaturing gradient gel electrophoresis) and sequencing of 16S rDNA sequences amplified from total consortium DNA revealed the presence of sequences related to those of Ureibacillus thermosphaericus, uncultured bacterium clone GC3, uncultured Clostridium sp. clone A1-3, Clostridium thermobutyricum, and Clostridium thermosuccinogenes in addition to the sequences identified from the cultured bacteria. The microbial community identified herein is a potential candidate consortium for the degradation of waste lignocellulosic biomass.
一个微生物联合体，命名为 WCS-6，是通过在静态条件下存在稻草的情况下连续传代培养而建立的。通过重量法测定，WCS-6 对滤纸、棉花和稻草的降解效率在 3 天培养后分别达到 99.0±0.7%、76.9±1.5%和 81.3±0.8%。从有氧条件下的 WCS-6 平板中获得了 9 个细菌分离株，其 16S rDNA 测序表明这些细菌与嗜热解淀粉芽孢杆菌 BTa、巴氏芽胞杆菌 SAFN-016、变形菌 S072、假单胞菌 taiwanensis CB-226、根瘤菌科 str. M100、芽孢杆菌 E53-10、β变形菌 HMD444、琥珀酸拟杆菌 4BON 和 Tepidiphilus margaritifer N2-214 有关。DGGE（变性梯度凝胶电泳）和从总联合体 DNA 扩增的 16S rDNA 序列测序显示，除了从培养细菌中鉴定出的序列外，还存在与 Ureibacillus thermosphaericus、未培养细菌克隆 GC3、未培养梭菌克隆 A1-3、嗜热丁酸梭菌和嗜热纤维梭菌有关的序列。本文鉴定的微生物群落是一种潜在的用于降解废木质纤维素生物质的候选联合体。
