Zhang Dongdong, Wang Yi, Zhang Chunfang, Zheng Dan, Guo Peng, Cui Zongjun
Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan 316021, P.R. China.
Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R. China.
J Microbiol Biotechnol. 2018 Feb 28;28(2):305-313. doi: 10.4014/jmb.1709.09036.
A microbial consortium, TMC7, was enriched for the degradation of natural lignocellulosic materials under high temperature. TMC7 degraded 79.7% of rice straw during 15 days of incubation at 65°C. Extracellular xylanase was effectively secreted and hemicellulose was mainly degraded in the early stage (first 3 days), whereas primary decomposition of cellulose was observed as of day 3. The optimal temperature and initial pH for extracellular xylanase activity and lignocellulose degradation were 65°C and between 7.0 and 9.0, respectively. Extracellular xylanase activity was maintained above 80% and 85% over a wide range of temperature (50-75°C) and pH values (6.0-11.0), respectively. likely had the largest contribution to lignocellulose conversion in TMC7 initially, and , and might have also been involved in the later phase. These results demonstrate the potential practical application of TMC7 for lignocellulosic biomass utilization in the biotechnological industry under hot and alkaline conditions.
一个微生物群落TMC7在高温下被富集用于天然木质纤维素材料的降解。在65°C下培养15天期间，TMC7降解了79.7%的稻草。细胞外木聚糖酶被有效分泌，半纤维素主要在早期阶段（前3天）被降解，而纤维素的主要分解从第3天开始观察到。细胞外木聚糖酶活性和木质纤维素降解的最佳温度和初始pH分别为65°C和7.0至9.0之间。细胞外木聚糖酶活性在较宽的温度范围（50 - 75°C）和pH值范围（6.0 - 11.0）内分别保持在80%和85%以上。最初可能对TMC7中木质纤维素转化贡献最大，并且 ， 和 可能也参与了后期阶段。这些结果证明了TMC7在热碱性条件下的生物技术产业中用于木质纤维素生物质利用的潜在实际应用。
