Megger Dominik A, Bracht Thilo, Meyer Helmut E, Sitek Barbara
Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany.
Biochim Biophys Acta. 2013 Aug;1834(8):1581-90. doi: 10.1016/j.bbapap.2013.04.001. Epub 2013 Apr 6.
Nowadays, proteomic studies no longer focus only on identifying as many proteins as possible in a given sample, but aiming for an accurate quantification of them. Especially in clinical proteomics, the investigation of variable protein expression profiles can yield useful information on pathological pathways or biomarkers and drug targets related to a particular disease. Over the time, many quantitative proteomic approaches have been established allowing researchers in the field of proteomics to refer to a comprehensive toolbox of different methodologies. In this review we will give an overview of different methods of quantitative proteomics with focus on label-free proteomics and its use in clinical proteomics.
如今，蛋白质组学研究不再仅仅专注于在给定样本中鉴定尽可能多的蛋白质，而是致力于对它们进行精确量化。特别是在临床蛋白质组学中，对可变蛋白质表达谱的研究可以产生有关与特定疾病相关的病理途径、生物标志物和药物靶点的有用信息。随着时间的推移，已经建立了许多定量蛋白质组学方法，使蛋白质组学领域的研究人员能够参考一个包含不同方法的综合工具箱。在本综述中，我们将概述定量蛋白质组学的不同方法，重点是无标记蛋白质组学及其在临床蛋白质组学中的应用。
