Basu Ananya A, Zhang Xiaoyu
Department of Chemistry, Northwestern University, Evanston, IL 60208.
Chemistry of Life Processes Institute, Northwestern University, Evanston, IL 60208.
Front Chem Biol. 2024;3. doi: 10.3389/fchbi.2024.1352676. Epub 2024 Jan 15.
The development of multiplexing technologies for proteomics has enabled the quantification of proteins on a global scale across samples with high confidence. In the covalent ligand discovery pipeline, quantitative proteomics can be used to establish selectivity profiles and provide critical mechanistic insight into the action of lead compounds. Current multiplexing systems allow for the analysis of up to eighteen samples in a single run, allowing proteomic analyses to match the pace of high-throughput covalent ligand discovery workflows. This review discusses several quantitative proteomic techniques and their applications in the field of covalent ligand discovery.
蛋白质组学多重分析技术的发展使得能够在全球范围内对样本中的蛋白质进行高可信度的定量分析。在共价配体发现流程中，定量蛋白质组学可用于建立选择性概况，并为先导化合物的作用提供关键的机制性见解。目前的多重分析系统允许在一次运行中分析多达18个样本，使蛋白质组学分析能够跟上高通量共价配体发现工作流程的步伐。本文综述了几种定量蛋白质组学技术及其在共价配体发现领域的应用。
