NIRDBS Romania, Institute of Biological Research, 48 Republicii Street, Cluj-Napoca, Romania.
Extremophiles. 2013 May;17(3):523-34. doi: 10.1007/s00792-013-0537-5. Epub 2013 Apr 9.
The diversity of archaea and bacteria was investigated in two slightly alkaline, mesophilic hot springs from the Western Plain of Romania. Phylogenetic analysis showed a low diversity of Archaea, only three Euryarchaeota taxa being detected: Methanomethylovorans thermophila, Methanomassiliicoccus luminyensis and Methanococcus aeolicus. Twelve major bacterial groups were identified, both springs being dominated by Cyanobacteria, Chloroflexi and Proteobacteria. While at the phylum/class-level the microbial mats share a similar biodiversity; at the species level the geothermal springs investigated seem to be colonized by specific consortia. The dominant taxa were filamentous heterocyst-containing Fischerella, at 45 °C and non-heterocyst Leptolyngbya and Geitlerinema, at 55 °C. Other bacterial taxa (Thauera sp., Methyloversatilis universalis, Pannonibacter phragmitetus, Polymorphum gilvum, Metallibacterium sp. and Spartobacteria) were observed for the first time in association with a geothermal habitat. Based on their bacterial diversity the two mats were clustered together with other similar habitats from Europe and part of Asia, most likely the water temperature playing a major role in the formation of specific microbial communities that colonize the investigated thermal springs.
本研究调查了罗马尼亚西部平原两处略碱性、嗜中温温泉中的古菌和细菌多样性。系统发育分析显示古菌多样性较低，仅检测到三种广古菌门分类群：嗜热甲烷甲基杆菌、亮发菌甲烷球菌和嗜热甲烷球菌。鉴定出 12 个主要的细菌类群，两个温泉均以蓝藻、绿弯菌门和变形菌门为主。虽然在门/纲水平上，微生物垫具有相似的生物多样性；但在种水平上，所研究的地热点似乎被特定的共生体定植。优势类群为含有异形胞的丝状 Fischerella，在 45°C 下，而非异形胞的 Leptolyngbya 和 Geitlerinema 在 55°C 下。其他细菌类群（Thauera sp.、Methyloversatilis universalis、Pannonibacter phragmitetus、Polymorphum gilvum、Metallibacterium sp. 和 Spartobacteria）首次在与地热栖息地相关的环境中被观察到。基于其细菌多样性，这两个垫与来自欧洲和亚洲部分地区的其他类似栖息地聚类在一起，最有可能是水温在形成定植于所研究地热点的特定微生物群落方面发挥了主要作用。
