Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, 310058, Hangzhou City, People's Republic of China.
Cell Tissue Res. 2013 Jun;352(3):573-83. doi: 10.1007/s00441-013-1609-7. Epub 2013 Apr 9.
A new concept for wound therapy is the initiation of the regeneration of epidermal and dermal layers with appendages for skin function recovery. Bone-marrow-derived mesenchymal and epidermal stem cells (BMSCs and SSCs) are hypothesized to be able to home toward or to be transplanted to wound sites for skin repair and regeneration, but this awaits confirmation by further experimental and clinical evidence. In this study, the influence of the transplantation of BMSCs and SSCs with porous gelatin-β-tricalcium phosphate sponge as scaffolds on wound re-epithelization, collagen synthesis, skin tensile strength recovery, and skin appendage regeneration has been investigated. The transplantation of BMSCs or SSCs significantly accelerates wound re-epithelization, stimulates dermal collagen synthesis, and exhibits the trend to enhance the tensile strength recovery of skin. Furthermore, regenerative features of BMSCs and SSCs have been identified in activating blood vessel and hair follicle formation, respectively. These results not only provide experimental evidence for the application of BMSCs and SSCs as promising therapeutics for clinical wound treatment, but also display their characteristics in activating distinct skin appendage regeneration, which might have novel applications in skin tissue engineering.
一种新的伤口治疗理念是启动表皮和真皮层的再生，形成附属物以恢复皮肤功能。骨髓间充质干细胞和表皮干细胞（BMSCs 和 SSCs）被假设能够向伤口部位归巢或移植，以进行皮肤修复和再生，但这需要进一步的实验和临床证据来证实。在这项研究中，研究了将 BMSCs 和 SSCs 与多孔明胶-β-磷酸三钙海绵作为支架移植到伤口部位对伤口再上皮化、胶原合成、皮肤拉伸强度恢复和皮肤附属物再生的影响。BMSCs 或 SSCs 的移植显著加速了伤口的再上皮化，刺激了真皮胶原的合成，并表现出增强皮肤拉伸强度恢复的趋势。此外，BMSCs 和 SSCs 的再生特征分别表现在激活血管和毛囊的形成上。这些结果不仅为 BMSCs 和 SSCs 作为有前途的临床伤口治疗治疗剂的应用提供了实验依据，而且还显示了它们在激活不同皮肤附属物再生方面的特征，这可能在皮肤组织工程中有新的应用。
