Hamaguchi Shigeto, Hirose Tomoya, Akeda Yukihiro, Matsumoto Naoya, Irisawa Taro, Seki Masafumi, Hosotsubo Hideo, Tasaki Osamu, Oishi Kazunori, Shimazu Takeshi, Tomono Kazunori
Division of Infection Control and Prevention, Osaka University Graduate School of Medicine, Osaka, Japan.
J Int Med Res. 2013 Feb;41(1):162-8. doi: 10.1177/0300060513475958. Epub 2013 Jan 23.
Neutrophils are able to form 'neutrophil extracellular traps' (NETs), which they use to trap and kill pathogens such as bacteria and fungi at the foci of infection. This observational study investigated the presence of NETs in the blood from critically ill patients and healthy volunteers.
Fluorescent triple-colour immunocytochemical analysis of blood smears collected from patients with systemic inflammatory response syndrome (SIRS; associated with various clinical conditions) who had been hospitalized in the intensive care unit, and healthy volunteers, was undertaken to identify NETs in the blood. Blood smears were stained for DNA, histone H1 and neutrophil elastase.
NETs were identified in 10 of 21 (47.6%) blood samples from the study group compared with none of the blood samples from eight healthy volunteers.
These data suggest that fluorescent triple-colour immunocytochemical staining of NETs in the blood could be used to simplify the early identification of critically ill patients with SIRS. Larger studies are required to clarify the pathophysiological role of NETs in this specific patient population.
中性粒细胞能够形成“中性粒细胞胞外诱捕网”（NETs），它们利用这些诱捕网在感染灶捕获并杀死诸如细菌和真菌等病原体。这项观察性研究调查了重症患者和健康志愿者血液中NETs的存在情况。
对入住重症监护病房的全身炎症反应综合征（SIRS；与多种临床病症相关）患者以及健康志愿者采集的血涂片进行荧光三色免疫细胞化学分析，以识别血液中的NETs。血涂片用DNA、组蛋白H1和中性粒细胞弹性蛋白酶进行染色。
研究组21份血液样本中有10份（47.6%）检测到NETs，而8名健康志愿者的血液样本均未检测到。
这些数据表明，血液中NETs的荧光三色免疫细胞化学染色可用于简化SIRS重症患者的早期识别。需要开展更大规模的研究来阐明NETs在这一特定患者群体中的病理生理作用。
