大戟叶提取物体外诱导白色念珠菌细胞多样性的透射电子显微镜研究
A transmission electron microscopy study of the diversity of Candida albicans cells induced by Euphorbia hirta L. leaf extract in vitro.
作者信息
Basma Abu Arra, Zuraini Zakaria, Sasidharan Sreenivasan
机构信息
Biology Division, School of Distance Education, Universiti Sains Malaysia, USM 11800, Pulau Pinang, Malaysia.
出版信息
Asian Pac J Trop Biomed. 2011 Jan;1(1):20-2. doi: 10.1016/S2221-1691(11)60062-2.
Abstract
OBJECTIVE
To determine the major changes in the microstructure of Candida albicans (C. albicans) after treatment with Euphorbia hirta (E. hirta) L. leaf extract.
METHODS
Transmission electron microscopy was used to study the ultrastructural changes caused by E. hirta extract on C. albicans cells at various exposure time.
RESULTS
It was found that the main abnormalities were the alterations in morphology, lysis and complete collapse of the yeast cells after 36 h of exposure to the extract. Whereas the control cultures showed a typical morphology of Candida with a uniform central density, typically structured nucleus, and a cytoplasm with several elements of endomembrane system and enveloped by a regular, intact cell wall.
CONCLUSIONS
The significant antifungal activity shown by this methanol extract of E. hirta L. suggests its potential against infections caused by C. albicans. The extract may be developed as an anticandidal agent.
摘要
目的
确定白念珠菌经大戟叶提取物处理后微观结构的主要变化。
方法
采用透射电子显微镜研究大戟叶提取物在不同暴露时间对白念珠菌细胞造成的超微结构变化。
结果
发现主要异常情况为暴露于提取物36小时后酵母细胞形态改变、溶解和完全崩溃。而对照培养物显示出念珠菌的典型形态，中央密度均匀，细胞核结构典型，细胞质中有几个内膜系统成分，并被规则、完整的细胞壁包裹。
结论
大戟叶甲醇提取物显示出显著的抗真菌活性，表明其对白色念珠菌引起的感染具有潜在作用。该提取物可能被开发为一种抗念珠菌剂。
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