体外、原位和体内研究金腰子种子提取物的抗真菌活性。

In vitro, in situ and in vivo studies on the anticandidal activity of Cassia fistula seed extract.

机构信息

Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.

出版信息

Molecules. 2012 Jun 7;17(6):6997-7009. doi: 10.3390/molecules17066997.

DOI:10.3390/molecules17066997

PMID:22678414

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6268276/

Abstract

Cassia fistula seeds have many therapeutic uses in traditional medicine practice. The present investigation was undertaken to demonstrate the anticandidal activity of the C. fistula seed extract at ultra-structural level through transmission electron microscope (TEM) and scanning electron microscope (SEM) observations. The effect of seed extract on the growth profile of the Candida albicans was examined via time-kill assays and in vivo efficacy of the extract was tested in an animal model. In addition, the anticandidal effect of seed extract was further evaluated by microscopic observations using SEM and TEM to determine any major alterations in the ultrastructure of C. albicans. The complete inhibition of C. albicans growth was shown by C. fistula seed extract at 6.25 mg/mL concentration. The time-kill assay suggested that C. fistula seed extract had completely inhibited the growth of C. albicans and also exhibited prolonged anti-yeast activity. The SEM and TEM observations carried out to distinguish the metamorphosis in the morphology of control and C. fistula seed extract-treated C. albicans cells revealed the notable effect on the outer cell wall and cytoplasmic content of the C. albicans and complete collapse of yeast cell exposed to seed extract at concentration 6.25 mg/mL at 36 h. The in vitro time-kill study performed using the leaf extract at 1/2, 1 or 2 times of the MIC significantly inhibited the yeast growth with a noticeable drop in optical density (OD) of yeast culture, thus confirming the fungicidal effect of the extract on C. albicans. In addition, in vivo antifungal activity studies on candidiasis in mice showed a 6-fold decrease in C. albicans in kidneys and blood samples in the groups of animals treated with the extract (2.5 g/kg body weight). The results suggested that the C. fistula seed extract possessed good anticandidal activity and is a potential candidate for the development of anticandidal agents.

摘要

木豆种子在传统医学实践中有许多治疗用途。本研究旨在通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）观察，证明木豆种子提取物在超微结构水平上的抗真菌活性。通过时间杀伤试验研究了种子提取物对白色念珠菌生长曲线的影响，并在动物模型中测试了提取物的体内功效。此外，通过 SEM 和 TEM 观察进行微观观察，进一步评估了种子提取物的抗真菌作用，以确定白色念珠菌超微结构的任何重大变化。木豆种子提取物在 6.25 mg/mL 浓度下完全抑制白色念珠菌的生长。时间杀伤试验表明，木豆种子提取物完全抑制了白色念珠菌的生长，并且表现出延长的抗酵母活性。进行 SEM 和 TEM 观察以区分对照和木豆种子提取物处理的白色念珠菌细胞的形态变化，结果表明，木豆种子提取物对白色念珠菌的细胞壁和细胞质内容物有显著影响，在 36 小时时，暴露于 6.25 mg/mL 浓度的种子提取物的酵母细胞完全崩溃。使用叶提取物在 MIC 的 1/2、1 或 2 倍进行的体外时间杀伤研究显著抑制了酵母生长，酵母培养物的光密度（OD）明显下降，从而证实了提取物对白色念珠菌的杀菌作用。此外，在小鼠念珠菌病的体内抗真菌活性研究中，在给予提取物（2.5 g/kg 体重）的动物组中，肾脏和血液样本中的白色念珠菌减少了 6 倍。结果表明，木豆种子提取物具有良好的抗真菌活性，是开发抗真菌药物的潜在候选药物。