Skolucka Nina, Daczewska Malgorzata, Saczko Jolanta, Chwilkowska Agnieszka, Choromanska Anna, Kotulska Malgorzata, Kaminska Iwona, Kulbacka Julita
Department of Medical Biochemistry, Medical University, Chalubinskiego 10 St., 50-368 Wroclaw, Poland.
Asian Pac J Trop Biomed. 2011 Apr;1(2):94-8. doi: 10.1016/S2221-1691(11)60003-8.
To estimate electroporation (EP) influence on malignant and normal cells.
Two cell lines including human malignant melanoma (Me-45) and normal human gingival fibroblast (HGFs) were used. EP parameters were the following: 250, 1 000, 1 750, 2 500 V/cm; 50 µs by 5 impulses for every case. The viability of cells after EP was estimated by MTT assay. The ultrastructural analysis was observed by transmission electron microscope (Zeiss EM 900).
In the current study we observed the intracellular effect following EP on Me-45 and HGF cells. At the conditions applied, we did not observe any significant damage of mitochondrial activity in both cell lines treated by EP. Conversely, we showed that EP in some conditions can stimulate cells to proliferation. Some changes induced by EP were only visible in electron microscopy. In fibroblast cells we observed significant changes in lower parameters of EP (250 and 1 000 V/cm). After applying higher electric field intensities (2 500 V/cm) we detected many vacuoles, myelin-like bodies and swallowed endoplasmic reticulum. In melanoma cells such strong pathological modifications after EP were not observed, in comparison with control cells. The ultrastructure of both treated cell lines was changed according to the applied parameters of EP.
We can claim that EP conditions are cell line dependent. In terms of the intracellular morphology, human fibroblasts are more sensitive to electric field as compared with melanoma cells. Optimal conditions should be determined for each cell line. Summarizing our study, we can conclude that EP is not an invasive method for human normal and malignant cells. This technique can be safely applied in chemotherapy for delivering drugs into tumor cells.
评估电穿孔（EP）对恶性细胞和正常细胞的影响。
使用了两种细胞系，包括人恶性黑色素瘤（Me - 45）和人正常牙龈成纤维细胞（HGFs）。EP参数如下：250、1000、1750、2500 V/cm；每种情况均为50 μs，5个脉冲。通过MTT法评估EP处理后细胞的活力。通过透射电子显微镜（蔡司EM 900）进行超微结构分析。
在本研究中，我们观察了EP处理后Me - 45和HGF细胞的细胞内效应。在所应用的条件下，我们未观察到EP处理的两种细胞系中线粒体活性有任何显著损伤。相反，我们表明在某些条件下EP可刺激细胞增殖。EP诱导的一些变化仅在电子显微镜下可见。在成纤维细胞中，我们观察到较低EP参数（250和1000 V/cm）时有显著变化。施加更高电场强度（2500 V/cm）后，我们检测到许多空泡、髓鞘样小体和扩张的内质网。与对照细胞相比，在黑色素瘤细胞中未观察到EP后如此强烈的病理改变。两种处理细胞系的超微结构根据所应用的EP参数而改变。
我们可以认为EP条件依赖于细胞系。就细胞内形态而言，人成纤维细胞比黑色素瘤细胞对电场更敏感。应为每种细胞系确定最佳条件。总结我们的研究，我们可以得出结论，EP对人正常细胞和恶性细胞不是一种侵入性方法。该技术可安全地应用于化疗中向肿瘤细胞递送药物。
