• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

一种用于牙龈增厚的简单且成熟的人牙龈成纤维细胞组织培养方法。

A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.

作者信息

Saczko Jolanta, Dominiak Marzena, Kulbacka Julita, Chwiłkowska Agnieszka, Krawczykowska Honorata

机构信息

Department of Medical Biochemistry, Wroclaw Medical University, Wrocław, Poland.

出版信息

Folia Histochem Cytobiol. 2008;46(1):117-9. doi: 10.2478/v10042-008-0017-4.

DOI:10.2478/v10042-008-0017-4
PMID:18296274
Abstract

Recent advances in tissue engineering technology suggest its application in different medical fields, including periodontology. There are some reports of new non-enzymatic methods of isolating human gingival fibroblast for short-time cultivation in vitro to be used in autologous gingival augmentation. The aim of this study was to obtain a simple and established method of culturing human gingival fibroblasts. The authors developed a recurrent method that can be successfully used in autologous gingival augmentation.

摘要

组织工程技术的最新进展表明其可应用于包括牙周病学在内的不同医学领域。有一些关于分离人牙龈成纤维细胞的新型非酶法的报道,用于体外短期培养以用于自体牙龈增大术。本研究的目的是获得一种简单且成熟的人牙龈成纤维细胞培养方法。作者开发了一种可成功用于自体牙龈增大术的重复方法。

相似文献

1
A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.一种用于牙龈增厚的简单且成熟的人牙龈成纤维细胞组织培养方法。
Folia Histochem Cytobiol. 2008;46(1):117-9. doi: 10.2478/v10042-008-0017-4.
2
Culturing primary human gingival epithelial cells: comparison of two isolation techniques.原代人牙龈上皮细胞的培养:两种分离技术的比较
J Craniomaxillofac Surg. 2001 Aug;29(4):224-31. doi: 10.1054/jcms.2001.0229.
3
Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.
J Periodontal Res. 1999 Aug;34(6):323-8. doi: 10.1111/j.1600-0765.1999.tb02260.x.
4
A clinical and histologic evaluation of gingival fibroblasts seeding on a chitosan-based scaffold and its effect on the width of keratinized gingiva in dogs.一种壳聚糖基支架上种植牙龈成纤维细胞的临床和组织学评价及其对犬角化龈宽度的影响。
J Periodontol. 2011 Sep;82(9):1367-75. doi: 10.1902/jop.2011.100604. Epub 2011 Feb 2.
5
Culture of gingival fibroblasts on bioabsorbable regenerative materials in vitro.体外生物可吸收再生材料上牙龈成纤维细胞的培养
J Periodontol. 1999 Oct;70(10):1234-9. doi: 10.1902/jop.1999.70.10.1234.
6
Fabrication of cultured oral gingiva by tissue engineering techniques without materials of animal origin.采用无动物源材料的组织工程技术制备培养的口腔牙龈。
J Periodontol. 2006 Apr;77(4):672-7. doi: 10.1902/jop.2006.050223.
7
The use of a biostatic fascia lata thigh allograft as a scaffold for autologous human culture of fibroblasts--An in vitro study.使用生物静态阔筋膜张肌同种异体移植物作为人成纤维细胞自体培养的支架——一项体外研究。
Ann Anat. 2015 May;199:104-8. doi: 10.1016/j.aanat.2014.08.003. Epub 2014 Sep 20.
8
Effects of titanium surface topography on morphology and in vitro activity of human gingival fibroblasts.钛表面形貌对人牙龈成纤维细胞形态及体外活性的影响。
Minerva Stomatol. 2013 Jul-Aug;62(7-8):267-80.
9
[Primary culture of human gingival tissue cells in vitro].[人牙龈组织细胞的体外原代培养]
Zhonghua Ya Yi Xue Hui Za Zhi. 1991 Sep;10(3):88-97.
10
In vitro evaluation of acellular dermal matrix as a three-dimensional scaffold for gingival fibroblasts seeding.体外评价脱细胞真皮基质作为牙龈成纤维细胞种植的三维支架。
J Periodontol. 2011 Feb;82(2):293-301. doi: 10.1902/jop.2010.100121. Epub 2010 Sep 2.

引用本文的文献

1
Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study.人脱落乳牙干细胞分泌组与也门酸枣蜜联合对细胞活力和迁移的影响:一项体外研究
BDJ Open. 2024 Mar 13;10(1):21. doi: 10.1038/s41405-024-00197-5.
2
Evaluating the Translucency, Surface Roughness, and Cytotoxicity of a PMMA Acrylic Denture Base Reinforced with Bioactive Glasses.评估生物活性玻璃增强聚甲基丙烯酸甲酯(PMMA)丙烯酸义齿基托的透明度、表面粗糙度和细胞毒性。
J Funct Biomater. 2023 Dec 31;15(1):16. doi: 10.3390/jfb15010016.
3
Cell-based therapy in the management of Class III Miller's recession - A case report with 45-month follow-up.
基于细胞的疗法治疗Ⅲ级米勒氏退缩——一例随访45个月的病例报告
J Indian Soc Periodontol. 2023 Jul-Aug;27(4):437-442. doi: 10.4103/jisp.jisp_436_22. Epub 2023 Jul 1.
4
Vitamin C influences antioxidative, anti-inflammatory and wound healing markers in smokers' gingival fibroblasts .维生素C影响吸烟者牙龈成纤维细胞中的抗氧化、抗炎和伤口愈合标志物。
Saudi Dent J. 2023 May;35(4):337-344. doi: 10.1016/j.sdentj.2023.03.009. Epub 2023 Mar 27.
5
Feldspar-Modified Methacrylic Composite for Fabrication of Prosthetic Teeth.用于制造假牙的长石改性甲基丙烯酸复合材料。
Materials (Basel). 2023 May 11;16(10):3674. doi: 10.3390/ma16103674.
6
Mechanical Properties and Biocompatibility of 3D Printing Acrylic Material with Bioactive Components.具有生物活性成分的3D打印丙烯酸材料的力学性能和生物相容性
J Funct Biomater. 2022 Dec 23;14(1):13. doi: 10.3390/jfb14010013.
7
Influence of surface modifications of a nanostructured implant on osseointegration capacity - preliminary study.纳米结构植入物表面改性对骨整合能力的影响——初步研究
RSC Adv. 2018 Apr 24;8(28):15533-15546. doi: 10.1039/c8ra01625a. eCollection 2018 Apr 23.
8
Mechanical Properties, Cytotoxicity, and Fluoride Ion Release Capacity of Bioactive Glass-Modified Methacrylate Resin Used in Three-Dimensional Printing Technology.三维打印技术中使用的生物活性玻璃改性甲基丙烯酸酯树脂的力学性能、细胞毒性和氟离子释放能力
Materials (Basel). 2022 Feb 1;15(3):1133. doi: 10.3390/ma15031133.
9
Antiradical Properties of N-Oxide Surfactants-Two in One.N-氧化物表面活性剂的抗氧化特性——一举两得。
Int J Mol Sci. 2021 Jul 27;22(15):8040. doi: 10.3390/ijms22158040.
10
Polymeric Nanocarriers with Luminescent Colloidal Nanoplatelets as Hydrophilic and Non-Toxic Two-Photon Bioimaging Agents.具有发光胶体纳米片的聚合物纳米载体作为亲水性和无毒的双光子生物成像剂。
Int J Nanomedicine. 2021 May 27;16:3649-3660. doi: 10.2147/IJN.S298300. eCollection 2021.