Tirupathi Rao G, Suresh Babu K, Ujwal Kumar J, Sujana P, Raoa A Veerabhadr, Sreedhar A S
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
Asian Pac J Trop Biomed. 2011 Aug;1(4):298-305. doi: 10.1016/S2221-1691(11)60047-6.
To examine the anti-bacterial activity of leaf extracts of Morus alba L. (Moraceae) and Piper betel L. (Piperaceae), and seed extracts of Bombax ceiba L. (Borabacaceae).
We have partially purified plant extracts by solvent extraction method, and evaluated the effect of individual fractions on bacterial growth using Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) bacterial strains.
Compared with Morus and Bombax fractions, Piper fractions showed significant growth inhibition on all the three types of bacteria studied. The EtOAc-hexane fractions of Piper leaves exhibited significant anti-bacterial activity with minimum inhibitory concentrations (MIC) of 50 µg/mL culture against both gram-positive and gram-negative bacteria. The EtOAc-fractions I, II, and IV inhibited bacterial colony formation on soft agar in addition to growth inhibition. A combination treatment of piper fractions with ampicillin resulted in significant growth inhibition in E. coli and P. aeruginosa, and combination with anticancer drug geldanamycin (2µg/mL) showed selective growth inhibition against P. aeruginosa and S. aureus. Three major compounds, i.e., eugenol, 3-hexene-ol and stigmasterol, were primarily identified from Piper betel leaf extractions. Among the individual compounds, eugenol treatment showed improved growth inhibition compared with stigmasterol and 3-hexene-ol.
We are reporting potential anti-bacterial compounds from Piper betel against both gram-positive and gram-negative bacteria either alone or in combination with drug treatment.
研究桑科植物桑（Morus alba L.）叶提取物、胡椒科植物蒌叶（Piper betel L.）叶提取物以及木棉科植物木棉（Bombax ceiba L.）种子提取物的抗菌活性。
采用溶剂萃取法对植物提取物进行部分纯化，并使用大肠杆菌（Escherichia coli，E. coli）、铜绿假单胞菌（Pseudomonas aeruginosa，P. aeruginosa）和金黄色葡萄球菌（Staphylococcus aureus，S. aureus）菌株评估各组分对细菌生长的影响。
与桑和木棉的组分相比，胡椒的组分对所研究的三种细菌均表现出显著的生长抑制作用。胡椒叶的乙酸乙酯 - 己烷组分对革兰氏阳性菌和革兰氏阴性菌均具有显著的抗菌活性，最低抑菌浓度（MIC）为50 μg/mL培养物。除生长抑制外，胡椒叶的乙酸乙酯组分I、II和IV还抑制软琼脂上的细菌菌落形成。胡椒组分与氨苄青霉素联合处理对大肠杆菌和铜绿假单胞菌有显著的生长抑制作用，与抗癌药物格尔德霉素（2 μg/mL）联合处理对铜绿假单胞菌和金黄色葡萄球菌有选择性生长抑制作用。从蒌叶提取物中初步鉴定出三种主要化合物，即丁香酚、3 - 己烯醇和豆甾醇。在各单一化合物中，丁香酚处理比豆甾醇和3 - 己烯醇表现出更好的生长抑制作用。
我们报道了蒌叶中具有潜在抗菌活性的化合物，其对革兰氏阳性菌和革兰氏阴性菌均有作用，可单独或与药物联合使用。
