Meenupriya J, Thangaraj M
Department of Biotechnology, Sathyabama University, Chennai-119, India.
Asian Pac J Trop Biomed. 2011 Oct;1(5):376-80. doi: 10.1016/S2221-1691(11)60083-X.
To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus (A. ochraceus) MP2 fungi.
The anti bacterial activity of marine sponge derived fungi A. ochraceus MP2 was thoroughly investigated against antagonistic human pathogens. The optimum inhibitory concentration of the fungi in the elite solvent was also determined. The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis. The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer (ITS) region of 5.8s rRNA using specific ITS primer. The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK.
Three bioactive compounds were characterized to reveal their identity, chemical formula and structure. The first elutant was identified asα- Campholene aldehyde with chemical formula C10 H16 O and molecular weight 152 Da. The second elutant was identified as Lucenin-2 and chemical formula C27 H30 O16 and molecular weight 610 Da. The third elutant was identified as 6-Ethyloct- 3-yl- 2- ethylhexyl ester with Chemical formula C26 H42 O4 with molecular weight 418 Da.
The isolated compounds showed significant antimicrobial activity against potential human pathogens. Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.
从赭曲霉（Aspergillus ochraceus）MP2真菌中分离并鉴定具有生物活性的次生代谢产物。
深入研究海洋海绵衍生真菌赭曲霉MP2对人类致病拮抗菌的抗菌活性。还测定了该真菌在优质溶剂中的最佳抑菌浓度。对显示出良好抗菌活性的有前景的提取物进行进一步的分析分离，以获得单个不同的代谢产物，并通过气相色谱 - 质谱联用仪（GC - MS）仪器分析进一步鉴定洗脱液。通过分离其基因组DNA并使用特异性ITS引物扩增5.8s rRNA的内部转录间隔区（ITS）来完成优质真菌菌株的分子表征。通过同源性搜索工具证明该菌株的新颖性，并将优质序列提交到GENBANK。
鉴定出三种生物活性化合物，揭示了它们的身份、化学式和结构。第一种洗脱液被鉴定为α - 莰烯醛，化学式为C10 H16 O，分子量为152 Da。第二种洗脱液被鉴定为卢森宁 - 2，化学式为C27 H30 O16，分子量为610 Da。第三种洗脱液被鉴定为6 - 乙基辛 - 3 - 基 - 2 - 乙基己酯，化学式为C26 H42 O4，分子量为418 Da。
分离出的化合物对潜在的人类病原体显示出显著的抗菌活性。微生物次生代谢产物是具有精巧结构和强大生物活性的化合物的重要来源。
