Department of Coastal Resources Management, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50275, Indonesia.
Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50275, Indonesia.
Mar Drugs. 2020 Apr 24;18(4):227. doi: 10.3390/md18040227.
Sponge-associated fungi are attractive targets for the isolation of bioactive natural products with different pharmaceutical purposes. In this investigation, 20 fungi were isolated from 10 different sponge specimens. One isolate, the fungus strain WK-P9, showed activity against JH642 when cultivated in malt extract medium. One new and three known citrinin derivatives were isolated from the extract of this fungus. The structures were elucidated by 1D and 2D NMR spectroscopy, as well as LC-HRMS. Their antibacterial activity against a set of common human pathogenic bacteria and fungi was tested. Compound showed moderate activity against ATCC607 with a minimum inhibitory concentration (MIC) of 32 µg/mL. Compound exhibited moderate growth inhibition against JH642, DSM32, and ATCC607 with MICs of 16, 16, and 32 µg/mL, respectively. Furthermore, weak activities of 64 µg/mL against DSM10 and ATCC25923 were observed for compound .
海绵共生真菌是分离具有不同药用目的生物活性天然产物的有吸引力的目标。在这项研究中，从 10 种不同的海绵标本中分离出 20 种真菌。一种分离株，真菌菌株 WK-P9，在麦芽提取物培养基中培养时对 JH642 表现出活性。从该真菌的提取物中分离出一种新的和三种已知的桔青霉素衍生物。通过 1D 和 2D NMR 光谱以及 LC-HRMS 确定了它们的结构。测试了它们对一组常见的人类致病细菌和真菌的抗菌活性。化合物 对 ATCC607 表现出中等活性，最小抑菌浓度（MIC）为 32 µg/mL。化合物 对 JH642、DSM32 和 ATCC607 的生长抑制作用中等，MIC 分别为 16、16 和 32 µg/mL。此外，化合物 对 DSM10 和 ATCC25923 的活性较弱，MIC 分别为 64 µg/mL。
