Jain Mahendra, Kapadia Rakhee, Jadeja Ravirajsinh N, Thounaojam Menaka C, Devkar Ranjitsinh V, Mishra S H
Herbal Drug Technology Laboratory, Pharmacy Department, Faculty of Technology and Engineering, The M. S. University of Baroda, Kalabhavan, Vadodara-390001, Gujarat, India.
Asian Pac J Trop Biomed. 2011 Dec;1(6):443-7. doi: 10.1016/S2221-1691(11)60097-X.
To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia).
Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The % purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 µg/mL). Based on the inhibitory concentration (IC50) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 µg/mL) for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT).
Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations.
It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.
评估毛叶番荔枝（Feronia limonia，F. limonia）叶提取物、馏分或分离化合物的细胞毒性和肝保护潜力。
通过薄层色谱法和光谱分析对提取物、馏分或化合物进行定性植物化学分析。采用分析型高效液相色谱法测定化合物的纯度百分比。分别评估提取物、馏分或化合物的细胞毒性作用（10、20、100、250、500、750和1000 μg/mL）。根据细胞活力测定获得的抑制浓度（IC50），评估提取物、馏分或分离化合物的分级浓度（10、20、50、100、200 μg/mL）对四氯化碳诱导的肝毒性的肝保护潜力，通过监测血清谷丙转氨酶（SGPT）和血清谷草转氨酶（SGOT）的活性水平来评估。
结果表明，与石油醚提取物相比，毛叶番荔枝甲醇提取物无毒且具有肝保护作用。甲醇提取物的丙酮馏分也表现出类似性质，但随后的两个馏分具有细胞毒性。然而，从甲醇提取物的倒数第二个馏分中分离出的纯化合物无毒且具有肝保护作用。生化研究（SGOT、SGPT）进一步证实了这些细胞学观察结果。
从本研究可以得出结论，毛叶番荔枝甲醇提取物、一些馏分和纯分离化合物在此处表现出肝保护活性。然而，倒数第二个馏分中记录的细胞毒性以及纯化合物结构细节的研究值得进一步探讨。
