药用植物根茎的细胞毒性。
Cytotoxicity of the rhizome of medicinal plants.
作者信息
Hossain Shakhawoat, Kader Golam, Nikkon Farjana, Yeasmin Tanzima
机构信息
Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh.
出版信息
Asian Pac J Trop Biomed. 2012 Feb;2(2):125-7. doi: 10.1016/S2221-1691(11)60205-0.
OBJECTIVE
To investigate the cytotoxicity of the crude ethanol extract of the rhizome of Zingiber zerumbet (Z. zerumbet) (L) Smith. and Curcuma zedoaria (C. zedoaria) Rosc. against Artemia salina Leach.
METHODS
Fresh rhizomes of Z. zerumbet (L) Smith. and C. zedoaria Rosc. were extracted separately in cold with ethanol (2.5 L) and after concentration a brownish syrupy suspension of ethanol extracts of Z. zerumbet (L) Smith. and C. zedoaria Rosc. was obtained. The cytotoxic effect of the crude ethanol extracts of both plants was determined by brine shrimp lethality bioassay.
RESULTS
Crude ethanol extracts of the rhizome of Z. zerumbet (L) Smith. showed the highest cytotoxicity (LC50 was 1.24 µg/mL) against brine shrimp nauplii as compared with C. zedoaria Rosc. (LC50 was 33.593 µg/mL) after 24 h of exposure.
CONCLUSIONS
It can be concluded that the rhizome of Z. zerumbet (L) Smith. and C. zedoaria Rosc. can be used as a source of cytotoxic agent.
目的
研究红球姜（Zingiber zerumbet (L) Smith.）和莪术（Curcuma zedoaria Rosc.）根茎的乙醇粗提取物对卤虫（Artemia salina Leach.）的细胞毒性。
方法
分别用乙醇（2.5 L）对新鲜的红球姜（Zingiber zerumbet (L) Smith.）和莪术（Curcuma zedoaria Rosc.）根茎进行冷提取，浓缩后得到红球姜（Zingiber zerumbet (L) Smith.）和莪术（Curcuma zedoaria Rosc.）乙醇提取物的棕褐色糖浆状悬浮液。通过卤虫致死生物测定法测定两种植物乙醇粗提取物的细胞毒性。
结果
暴露24小时后，与莪术（Curcuma zedoaria Rosc.）（LC50为33.593 μg/mL）相比，红球姜（Zingiber zerumbet (L) Smith.）根茎的乙醇粗提取物对卤虫无节幼体显示出最高的细胞毒性（LC50为1.24 μg/mL）。
结论
可以得出结论，红球姜（Zingiber zerumbet (L) Smith.）和莪术（Curcuma zedoaria Rosc.）的根茎可作为细胞毒性剂的来源。
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