Akher Sayed Abdul, Wang Kevin Yueju, Hall Kylie, Hunpatin Oluwaseyi Setonji, Shan Muhammad, Zhang Zenglin, Guo Yongfeng
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2025 Jun 9;26(12):5510. doi: 10.3390/ijms26125510.
Plant Molecular Farming (PMF) capitalizes on the unique properties of plants as bioreactors to efficiently produce valuable proteins, pharmaceuticals, and enzymes. This review emphasizes the critical role of transient expression systems, particularly in , due to its susceptibility to various pathogens. Viral vector-based transient expression has proven essential during health emergencies like COVID-19, enabling rapid recombinant protein production. The review also evaluates different transient expression platforms and highlights their applications in biopharmaceutical production, education, synthetic biology, and gene editing. Advances in viral vector modification, hydroponics, and Controlled Environment Agriculture (CEA) are presented as transformative innovations enhancing scalability and regulatory compliance. Furthermore, glycoengineering advancements broaden the range of producible biopharmaceuticals, improving global medication access. By exploring these advancements, this review underscores the vast potential of transient expression systems to meet dynamic scientific and market demands, positioning PMF as a vital component in modern biotechnology.
植物分子农场（PMF）利用植物作为生物反应器的独特特性，高效生产有价值的蛋白质、药物和酶。本综述强调了瞬时表达系统的关键作用，特别是在[具体内容缺失]方面，因为它易受各种病原体的影响。基于病毒载体的瞬时表达在 COVID - 19 等健康紧急情况期间已被证明至关重要，能够实现重组蛋白的快速生产。该综述还评估了不同的瞬时表达平台，并突出了它们在生物制药生产、教育、合成生物学和基因编辑中的应用。病毒载体修饰、水培和可控环境农业（CEA）方面的进展被视为变革性创新，可增强可扩展性并符合监管要求。此外，糖基工程的进展拓宽了可生产生物制药的范围，改善了全球药物可及性。通过探索这些进展，本综述强调了瞬时表达系统满足动态科学和市场需求的巨大潜力，将植物分子农场定位为现代生物技术的重要组成部分。
