洋葱中槲皮素对链脲佐菌素诱导的糖尿病大鼠醛氧化酶（氧化型低密度脂蛋白）及肝细胞凋亡的抗氧化作用
Anti-oxidative role of quercetin derived from Allium cepa on aldehyde oxidase (OX-LDL) and hepatocytes apoptosis in streptozotocin-induced diabetic rat.
作者信息
Bakhshaeshi Mina, Khaki Arash, Fathiazad Fatemeh, Khaki Amir Afshin, Ghadamkheir Elham
机构信息
Member of Medicine Scientific Association, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
出版信息
Asian Pac J Trop Biomed. 2012 Jul;2(7):528-31. doi: 10.1016/S2221-1691(12)60090-2.
OBJECTIVE
To study the role of Quercetin in streptozotocin-induced diabetes in rats.
METHODS
Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group received 15 mg/kg (IP) QR, (n=10), and diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus 15 mg/kg QR, daily for 4 weeks, respectively; however, the control group just received an equal volume of distilled water daily (IP). Diabetes was induced by a single (IP) injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. Twenty-eight days after inducing diabetic, 5 mL blood were collected for TAC, MDA and Ox-LDL levels and liver tissues of rat in whole groups were removed then prepared for apoptosis analysis by Tunel method.
RESULTS
Apoptotic cells significantly decreased in group that has received 15 mg/kg (IP) Quercetin (P<0.05) in comparison to experimental groups (P<0.05).
CONCLUSIONS
Since in our study 15 mg/kg (IP) Quercetin have significantly Preventive effect on liver cells damages by reducing number of apoptotic cells in Liver, so it seems that using it can be effective for treatment in diabetic rat.
目的
研究槲皮素在链脲佐菌素诱导的大鼠糖尿病中的作用。
方法
将40只雄性Wistar大鼠分为三组，对照组（n = 10）和槲皮素（QR）组腹腔注射15 mg/kg QR（n = 10），糖尿病组腹腔注射55 mg/kg链脲佐菌素（STZ）（n = 20），该组再分为两组，每组10只，即STZ组和治疗组。治疗组腹腔注射55 mg/kg STZ加15 mg/kg QR，每天一次，共4周；然而，对照组每天仅腹腔注射等量蒸馏水。通过单次腹腔注射链脲佐菌素（55 mg/kg）诱导糖尿病。动物饲养在标准条件下。诱导糖尿病28天后，采集5 mL血液检测总抗氧化能力（TAC）、丙二醛（MDA）和氧化低密度脂蛋白（Ox-LDL）水平，然后取出全组大鼠的肝脏组织，采用Tunel法进行凋亡分析。
结果
与实验组相比，接受15 mg/kg腹腔注射槲皮素的组凋亡细胞显著减少（P<0.05）。
结论
由于在我们的研究中，15 mg/kg腹腔注射槲皮素通过减少肝脏凋亡细胞数量对肝细胞损伤具有显著的预防作用，因此似乎使用它对糖尿病大鼠的治疗可能有效。
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