Mochida Naoko, Matsumura Yoko, Kitabatake Masahiro, Ito Toshihiro, Kayano Shin-Ichi, Kikuzaki Hiroe
Department of Food Science & Nutrition, School of Humanities & Science, Nara Women's University, Nara 630-8506, Japan.
Department of Health and Nutrition, Faculty of Health Science, Kio University, Kitakatsuragi-gun, Nara 635-0832, Japan.
Antioxidants (Basel). 2022 Aug 11;11(8):1555. doi: 10.3390/antiox11081555.
Oxidative stress causes the progression of diabetes and its complications; thus, maintaining the balance between reactive oxygen species produced by hyperglycemia and the antioxidant defense system is important. We herein examined the antioxidant potential of non-extractable fractions of dried persimmon (NEP) against oxidative stress in diabetic rats. Rats with streptozotocin-induced type 1 diabetes (50 mg/kg body weight) were administered NEP for 9 weeks. Antioxidant enzyme activities and concentration of antioxidants in liver tissues were analyzed with a microplate reader. Extensor digitorum longus (EDL) and soleus muscle fibers were stained with succinate dehydrogenase and muscle fiber sizes were measured. The administration of NEP increased the body weight of diabetes rats. Regarding antioxidant activities, the oxygen radical absorbance capacity and superoxide dismutase activity in liver tissues significantly increased. In addition, increases in glutathione peroxidase activity in liver tissues and reductions in the cross-sectional area of EDL muscle fibers were significantly suppressed. In these results, NEP improved the antioxidant defense system in the liver tissues of diabetic rats, in addition to attenuating of muscle fibers atrophy against oxidative damage induced by hyperglycemia.
氧化应激会导致糖尿病及其并发症的发展；因此，维持高血糖产生的活性氧与抗氧化防御系统之间的平衡至关重要。我们在此研究了柿饼不可提取部分（NEP）对糖尿病大鼠氧化应激的抗氧化潜力。用链脲佐菌素诱导1型糖尿病（50毫克/千克体重）的大鼠连续9周给予NEP。用酶标仪分析肝脏组织中的抗氧化酶活性和抗氧化剂浓度。用琥珀酸脱氢酶对趾长伸肌（EDL）和比目鱼肌纤维进行染色，并测量肌纤维大小。给予NEP增加了糖尿病大鼠的体重。关于抗氧化活性，肝脏组织中的氧自由基吸收能力和超氧化物歧化酶活性显著增加。此外，肝脏组织中谷胱甘肽过氧化物酶活性的增加以及EDL肌纤维横截面积的减少得到了显著抑制。这些结果表明，NEP除了减轻高血糖诱导的氧化损伤导致的肌纤维萎缩外，还改善了糖尿病大鼠肝脏组织中的抗氧化防御系统。
