Safi Sher Zaman, Waheed Yasir, Sadat Joharia, Salahuddin Sadia, Saeed Umar, Ashraf Muhammad
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan.
Asian Pac J Trop Biomed. 2012 Jul;2(7):532-6. doi: 10.1016/S2221-1691(12)60091-4.
To determine hepatitis C virus (HCV) genotypes and explore the associated risk factors in chronic HCV patients.
A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction (PCR) based detection out of which 112 chronic HCV patients [53 male (47.32%), 59 female (52.68%); mean age (43.76±16.40) years; mean BMI (23.8±3.9) kg] were enrolled in this study. The frequency of 6 HCV genotypes and associated risk factors were evaluated from five districts of North West Frontier Province (NWFP).
Genotype 3 was the most prevalent in 73 samples (65.17%) followed by genotype 1 in 24 (21.42%) and genotype 2 in 13 (11.60%) samples. Genotype 3 had significantly high prevalence (P=0.000 2). The results showed that 48 (42.85%) samples were infected with HCV 3a; 25 (22.32%) with 3b; 14 (12.50%) with 1a; 10 (8.92%) with 1b; 11 (9.82%) with 2a; 2 (1.78%) with 2b; and 2 were untypable. The distribution of HCV genotypes in Mardan, Charsadda, Peshawar, Sawabi and Nowshehra districts was different. Use of unsterile equipment for medication, barbers and previous history of hospitalization were the main risk factors for HCV transmission.
Genotype 3a and 3b, 1a, 1b and 2a are the common genotypes in NWFP. Genotype 4, 5, and 6 can not be found in a single sample. The level of awareness about various modes of transmission of HCV among the population is found to be very low.
确定丙型肝炎病毒（HCV）基因型，并探究慢性HCV患者的相关危险因素。
对116例慢性丙型肝炎患者进行基于聚合酶链反应（PCR）的检测，其中112例慢性HCV患者[53例男性（47.32%），59例女性（52.68%）；平均年龄（43.76±16.40）岁；平均体重指数（23.8±3.9）kg]纳入本研究。评估了西北边境省（NWFP）五个地区6种HCV基因型的频率及相关危险因素。
基因型3在73份样本中最为常见（65.17%），其次是基因型1，有24份（21.42%），基因型2有13份（11.60%）。基因型3的患病率显著较高（P = 0.000 2）。结果显示，48份（42.85%）样本感染HCV 3a；25份（22.32%）感染3b；14份（12.50%）感染1a；10份（8.92%）感染1b；11份（9.82%）感染2a；2份（1.78%）感染2b；2份无法分型。马尔丹、查尔萨达、白沙瓦、萨瓦比和瑙谢拉地区HCV基因型的分布有所不同。使用未消毒的医疗设备、理发工具以及既往住院史是HCV传播的主要危险因素。
基因型3a和3b、1a、1b和2a是NWFP地区的常见基因型。在单个样本中未发现基因型4、5和6。研究发现人群对HCV各种传播方式的知晓程度非常低。
