Kaplan O, van Zijl P C, Cohen J S
Medicine Branch, NCI, National Institutes of Health, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1990 Jun 15;169(2):383-90. doi: 10.1016/0006-291x(90)90343-l.
Combined analysis of both 1H and 31P NMR spectra of extracts of drug-sensitive and multidrug-resistant MCF-7 human breast cancer cells enabled quantitative comparisons between the concentrations of major metabolites, as well as of their precursors. In resistant cells high energy phosphorus compounds, phosphocreatine and ATP, and also their precursors, creatine and ADP, were elevated compared to the sensitive cells. In phospholipid metabolism, the sensitive cells showed higher phosphocholine and phosphoethanolamine concentrations than the resistant cells, but choline levels were similar. These results delineated the differences in control of metabolic pathways between drug-sensitive and drug-resistant cells.
对药物敏感和多药耐药的MCF-7人乳腺癌细胞提取物的1H和31P NMR光谱进行联合分析，能够对主要代谢物及其前体的浓度进行定量比较。与敏感细胞相比，耐药细胞中的高能磷化合物、磷酸肌酸和ATP及其前体肌酸和ADP含量升高。在磷脂代谢方面，敏感细胞中的磷酸胆碱和磷酸乙醇胺浓度高于耐药细胞，但胆碱水平相似。这些结果描绘了药物敏感细胞和耐药细胞在代谢途径控制上的差异。
